Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana.

Autor: Addo SO; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana. sethaddo40@gmail.com.; Department of Theoretical and Applied Biology, KNUST, Kumasi, Ghana. sethaddo40@gmail.com., Bentil RE; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Addae CA; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Baako BOA; Navrongo Health Research Centre, Upper East Region, Navrongo, Ghana., Yartey KN; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Ansah-Owusu J; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Tawiah-Mensah CNL; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Agbodzi B; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Kumordjie S; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Yeboah C; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Asoala V; Navrongo Health Research Centre, Upper East Region, Navrongo, Ghana., Dunford JC; Navy Entomology Center of Excellence, Jacksonville, FL, USA.; Uniformed Services University of Health Sciences, Bethesda, MD, USA.; School of Community and Environmental Health, Old Dominion University, Norfolk, VA, USA., Larbi JA; Department of Theoretical and Applied Biology, KNUST, Kumasi, Ghana., Baidoo PK; Department of Theoretical and Applied Biology, KNUST, Kumasi, Ghana., Wilson MD; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Diclaro JW 2nd; Navy Entomology Center of Excellence, Center for Disease Control and Prevention Detachment, Atlanta, Georgia., Dadzie SK; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana. SDadzie@noguchi.ug.edu.gh.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2024 Mar 06; Vol. 169 (3), pp. 62. Date of Electronic Publication: 2024 Mar 06.
DOI: 10.1007/s00705-024-05983-y
Abstrakt: Sampled ticks were screened for Crimean-Congo haemorrhagic fever virus (CCHFV) using an assay that targets the nucleoprotein gene region of the S segment, a conserved region of the CCHFV genome. Minimum infection rates of 0.34% and 0.10% were obtained when testing pools of Hyalomma rufipes and Amblyomma variegatum, respectively. Next-generation sequencing and phylogenetic analysis showed that the S and L segments of the CCHFV isolate clustered with those of similar isolates of genotype III. However, analysis of the M segment showed that reassortment had occurred, causing this segment to cluster with those of isolates of genotype I, providing the first evidence of such an occurrence in Ghana.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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