Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division.

Autor: Nußbaum P; Molecular Biology of Archaea, Microbiology, Faculty of Biology, University of Freiburg, Freiburg, Germany., Kureisaite-Ciziene D; MRC Laboratory of Molecular Biology, Cambridge, UK., Bellini D; MRC Laboratory of Molecular Biology, Cambridge, UK., van der Does C; Molecular Biology of Archaea, Microbiology, Faculty of Biology, University of Freiburg, Freiburg, Germany., Kojic M; Institute of Science and Technology Austria (ISTA), Klosterneuburg, Austria., Taib N; Evolutionary Biology of the Microbial Cell Laboratory, Institut Pasteur, Université Paris Cité, Paris, France.; Bioinformatics and Biostatistics Hub, Institut Pasteur, Université Paris Cité, Paris, France., Yeates A; MRC Laboratory of Molecular Biology, Cambridge, UK., Tourte M; Molecular Biology of Archaea, Microbiology, Faculty of Biology, University of Freiburg, Freiburg, Germany., Gribaldo S; Evolutionary Biology of the Microbial Cell Laboratory, Institut Pasteur, Université Paris Cité, Paris, France., Loose M; Institute of Science and Technology Austria (ISTA), Klosterneuburg, Austria., Löwe J; MRC Laboratory of Molecular Biology, Cambridge, UK., Albers SV; Molecular Biology of Archaea, Microbiology, Faculty of Biology, University of Freiburg, Freiburg, Germany. sonja.albers@biologie.uni-freiburg.de.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany. sonja.albers@biologie.uni-freiburg.de.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2024 Mar; Vol. 9 (3), pp. 698-711. Date of Electronic Publication: 2024 Mar 04.
DOI: 10.1038/s41564-024-01600-5
Abstrakt: Cell division in all domains of life requires the orchestration of many proteins, but in Archaea most of the machinery remains poorly characterized. Here we investigate the FtsZ-based cell division mechanism in Haloferax volcanii and find proteins containing photosynthetic reaction centre (PRC) barrel domains that play an essential role in archaeal cell division. We rename these proteins cell division protein B 1 (CdpB1) and CdpB2. Depletions and deletions in their respective genes cause severe cell division defects, generating drastically enlarged cells. Fluorescence microscopy of tagged FtsZ1, FtsZ2 and SepF in CdpB1 and CdpB2 mutant strains revealed an unusually disordered divisome that is not organized into a distinct ring-like structure. Biochemical analysis shows that SepF forms a tripartite complex with CdpB1/2 and crystal structures suggest that these two proteins might form filaments, possibly aligning SepF and the FtsZ2 ring during cell division. Overall our results indicate that PRC-domain proteins play essential roles in FtsZ-based cell division in Archaea.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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