Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry.

Autor: Deslignière E; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands., Yin VC; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. v.c.yin@uu.nl., Ebberink EHTM; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands., Rolland AD; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands., Barendregt A; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands., Wörner TP; Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany., Nagornov KO; Spectroswiss, Lausanne, Switzerland., Kozhinov AN; Spectroswiss, Lausanne, Switzerland., Fort KL; Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany., Tsybin YO; Spectroswiss, Lausanne, Switzerland., Makarov AA; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.; Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany., Heck AJR; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. a.j.r.heck@uu.nl.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2024 Apr; Vol. 21 (4), pp. 619-622. Date of Electronic Publication: 2024 Mar 05.
DOI: 10.1038/s41592-024-02207-8
Abstrakt: Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to enable mass analysis of even highly heterogeneous, high-mass macromolecular assemblies. For contemporary Orbitrap instruments, the accessible ion detection (recording) times are maximally ~1-2 s. Here by modifying a data acquisition method on an Orbitrap ultrahigh mass range mass spectrometer, we trapped and monitored individual (single) ions for up to 25 s, resulting in a corresponding and huge improvement in signal-to-noise ratio (×5 compared with 1 s), mass resolution (×25) and accuracy in charge and mass determination of Orbitrap-based charge detection mass spectrometry.
(© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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