Biocontrol of Alternaria alternata in cold-stored table grapes using psychrotrophic yeasts and bioactive compounds of natural sources.

Autor: Torres-Palazzolo C; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CCT Mendoza, Av. Ruiz Leal s/n Parque General San Martín, Mendoza, Argentina; Estación Experimental Agropecuaria Mendoza, Instituto Nacional de Tecnología Agropecuaria (EEA Mza INTA), San Martín 3853, Luján de Cuyo, Mendoza, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Alte Brown 500, Chacras de Coria, Luján de Cuyo, Mendoza, Argentina., Ferreyra S; Instituto de Biología Agrícola de Mendoza, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Cuyo, Almirante Brown 500, Chacras de Coria, Mendoza, Argentina., Iribas F; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Padre Jorge Contreras 1300, Ciudad de Mendoza, Mendoza, Argentina., Chimeno V; Estación Experimental Agropecuaria Mendoza, Instituto Nacional de Tecnología Agropecuaria (EEA Mza INTA), San Martín 3853, Luján de Cuyo, Mendoza, Argentina., Rojo MC; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CCT Mendoza, Av. Ruiz Leal s/n Parque General San Martín, Mendoza, Argentina; Estación Experimental Agropecuaria Mendoza, Instituto Nacional de Tecnología Agropecuaria (EEA Mza INTA), San Martín 3853, Luján de Cuyo, Mendoza, Argentina., Casalongue C; Instituto de Investigaciones Biológicas (IIB), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Consejo Nacional de Investigaciones Científicas y Técnicas, Funes 3250, B7600 Mar del Plata, Argentina., Fontana A; Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Alte Brown 500, Chacras de Coria, Luján de Cuyo, Mendoza, Argentina; Instituto de Biología Agrícola de Mendoza, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Cuyo, Almirante Brown 500, Chacras de Coria, Mendoza, Argentina., Combina M; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CCT Mendoza, Av. Ruiz Leal s/n Parque General San Martín, Mendoza, Argentina; Estación Experimental Agropecuaria Mendoza, Instituto Nacional de Tecnología Agropecuaria (EEA Mza INTA), San Martín 3853, Luján de Cuyo, Mendoza, Argentina., Ponsone ML; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CCT Mendoza, Av. Ruiz Leal s/n Parque General San Martín, Mendoza, Argentina; Estación Experimental Agropecuaria Mendoza, Instituto Nacional de Tecnología Agropecuaria (EEA Mza INTA), San Martín 3853, Luján de Cuyo, Mendoza, Argentina; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Padre Jorge Contreras 1300, Ciudad de Mendoza, Mendoza, Argentina. Electronic address: ponsone.lorena@inta.gob.ar.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2024 Apr 16; Vol. 415, pp. 110640. Date of Electronic Publication: 2024 Feb 27.
DOI: 10.1016/j.ijfoodmicro.2024.110640
Abstrakt: Alternaria alternata is a common fungal pathogen causing postharvest decay in table grapes. This study addressed the potential of autochthonous yeasts and bioactive compounds of natural sources to act as biocontrol agents (BCAs) against A. alternata in cold-stored table grapes. With this purpose, 19 yeast capable of growing at 0-1 °C were isolated from the surface of Red Globe table grapes. These isolates, along with the pre-isolated strain Metschnikowia pulcherrima RCM2, were evaluated as BCAs in wounded berries. From these results, six yeast isolates were pre-selected to be combined with bioactive compounds of natural sources, like phenolic compounds (PCs) of side streams of wine industry, including bunch stem extract (BSE) (5-25 %), and cane extract (CE) (5-25 %), and functional polysaccharides from shrimp waste such as chitosan (CH) (0.5 %). Then, the biocontrol efficacy of combined treatments beyond individual ones was compared. The results revealed that 4 yeast isolates, namely M. pulcherrima RCM2 and ULA146, and Aureobasidium pullulans FUL14 and FUL18, were the most effective. However, when combined with the natural bioactive compounds, their efficacy against A. alternata did not increase significantly. Notably, ULA146 and FUL18 demonstrated a biocontrol efficacy of 36-37 %, comparable to that of the treatment with commercial doses of SO 2 , which only showed a 27 % reduction in the lesion diameter. These findings highlight the potential of using psychrotrophic yeasts as BCAs against A. alternata in cold-stored table grapes. Combining these yeast strains with BSE, CE and CH did not increase BCAs efficacy against this pathogen at the concentrations tested. The development of effective biocontrol strategies for A. alternata could contribute to reducing reliance on chemically synthesized fungicides, promoting sustainable practices, aiming to improve the quality and safety of cold-stored table grapes.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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