Strain-Promoted Cycloadditions in Lipid Bilayers Triggered by Liposome Fusion.

Autor: Jumeaux C; Department of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, United Kingdom., Spicer CD; Department of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, United Kingdom.; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, 17177, Sweden.; Department of Chemistry and York Biomedical Research Institute, University of York, Heslington, YO10 5DD, United Kingdom., Charchar P; School of Engineering, RMIT University, Melbourne, Victoria, 3001, Australia., Howes PD; Department of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, United Kingdom.; Present Addresses: Department of Engineering and Design, School of Engineering and Informatics, University of Sussex, BN1 9RH, Brighton, United Kingdom., Holme MN; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, 17177, Sweden., Ma L; Department of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, United Kingdom.; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, 17177, Sweden., Rose NC; Department of Chemistry and York Biomedical Research Institute, University of York, Heslington, YO10 5DD, United Kingdom., Nabarro J; Department of Chemistry and York Biomedical Research Institute, University of York, Heslington, YO10 5DD, United Kingdom., Fascione MA; Department of Chemistry and York Biomedical Research Institute, University of York, Heslington, YO10 5DD, United Kingdom., Rashid MH; School of Engineering, RMIT University, Melbourne, Victoria, 3001, Australia.; Present Addresses: Department of Mathematics and Physics, North South University, Bashundhara, Dhaka, 1229, Bangladesh., Yarovsky I; School of Engineering, RMIT University, Melbourne, Victoria, 3001, Australia., Stevens MM; Department of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, United Kingdom.; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, 17177, Sweden.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2024 Apr 02; Vol. 63 (14), pp. e202314786. Date of Electronic Publication: 2024 Mar 04.
DOI: 10.1002/anie.202314786
Abstrakt: Due to the variety of roles served by the cell membrane, its composition and structure are complex, making it difficult to study. Bioorthogonal reactions, such as the strain promoted azide-alkyne cycloaddition (SPAAC), are powerful tools for exploring the function of biomolecules in their native environment but have been largely unexplored within the context of lipid bilayers. Here, we developed a new approach to study the SPAAC reaction in liposomal membranes using azide- and strained alkyne-functionalized Förster resonance energy transfer (FRET) dye pairs. This study represents the first characterization of the SPAAC reaction between diffusing molecules inside liposomal membranes. Potential applications of this work include in situ bioorthogonal labeling of membrane proteins, improved understanding of membrane dynamics and fluidity, and the generation of new probes for biosensing assays.
(© 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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