Histological analysis of (antral) follicle density in ovarian cortex tissue attached to stripped endometriomas.

Autor: de Koning R; Department of Gynaecology and Obstetrics, Leiden University Medical Centre, Leiden, The Netherlands. R.de_Koning@lumc.nl.; Endometriose Centrum, Haaglanden Medical Centre, Den Haag, The Netherlands. R.de_Koning@lumc.nl.; Nederlandse Endometriose Kliniek, Reinier de Graaf Hospital, Delft, The Netherlands. R.de_Koning@lumc.nl., Blikkendaal MD; Endometriose Centrum, Haaglanden Medical Centre, Den Haag, The Netherlands.; Nederlandse Endometriose Kliniek, Reinier de Graaf Hospital, Delft, The Netherlands., de Sousa Lopes SMC; Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands., van der Meeren LE; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands.; Department of Pathology, Erasmus Medical Centre, Rotterdam, The Netherlands., Cheng H; Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands., Jansen FW; Department of Gynaecology and Obstetrics, Leiden University Medical Centre, Leiden, The Netherlands., Lashley EELO; Department of Gynaecology and Obstetrics, Leiden University Medical Centre, Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2024 Apr; Vol. 41 (4), pp. 1067-1076. Date of Electronic Publication: 2024 Mar 05.
DOI: 10.1007/s10815-024-03058-0
Abstrakt: Purpose: When resecting endometriomas with the stripping technique, in the majority of cases, a thin line of adjacent ovarian cortex is attached to the endometrioma. In this study, we performed histological analysis to determine (antral) follicle density in the ovarian cortex tissue attached to stripped endometriomas and assessed patient- and surgical characteristics that could affect this.
Methods: Histological slides of previously removed endometriomas were assessed. Follicles in the attached ovarian tissue were classified according to maturation, and follicular density was determined. Immunofluorescent staining of antral follicles in a subset of endometriomas was also performed.
Results: In 90 out of 96 included endometriomas (93.7%), ovarian tissue attached to the cyst wall was observed. One thousand nine hundred forty-four follicles at different maturation stages were identified (3 follicles/mm 3 ). Follicle density was negatively associated with age (p < 0.001). Antral follicles (< 7-mm diameter) were present in the ovarian tissue attached to 35 endometriomas (36.5%) derived from younger patients compared to endometriomas where none were detected (30 versus 35 years, p = 0.003). Antral follicle density was 1 follicle/mm 3 . Based on immunofluorescence, healthy antral follicles were identified in two out of four examined endometriomas.
Conclusions: Ovarian tissue attached to stripped endometriomas holds potential as a non-invasive source for antral follicles. In theory, application of IVM could be an interesting alternative FP option in young patients with endometriomas who undergo cystectomy in order to transform the surgical collateral damage to a potential oocyte source. Our results encourage future research with fresh tissue to further assess the quality and potential of these follicles.
Trial Registration: Clinical Trials.gov Identifier: B21.055 (METC LDD), date of registration 12-08-2021, retrospectively registered.
(© 2024. The Author(s).)
Databáze: MEDLINE
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