| Autor: |
Vaziri AS; Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran 14115-111, Iran.; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany., Vasheghani-Farahani E; Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran 14115-111, Iran., Hosseinzadeh S; Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1968917313, Iran., Bagheri F; Biotechnology Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran 14115-111, Iran., Büchner M; Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany., Schubert DW; Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany., Boccaccini AR; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany. |
| Abstrakt: |
Genipin-cross-linked silk fibroin (SF) hydrogel is considered to be biocompatible and mechanically robust. However, its use remains a challenge for in situ forming applications due to its prolonged gelation process. In our attempt to facilitate the in situ fabrication of a genipin-mediated SF hydrogel, alginate dialdehyde (ADA) was utilized as a reinforcement template. Here, SF/ADA-based hydrogels with different compositions were synthesized covalently and ionically. Incorporating ADA into the SF hydrogel increased pore size (44.66-174.66 μm), porosity (61.59-80.40%), and the equilibrium swelling degree (7.60-30.17). Moreover, a wide range of storage modulus and compressive modulus were obtained by adjusting the proportions of SF and ADA networks within the hydrogel. The in vitro cell analysis using preosteoblast cells (MC3T3-E1) demonstrated the cytocompatibility of all hydrogels. Overall, the covalently and ionically cross-linked SF/ADA hydrogel represents a promising solution for in situ forming hydrogels for applications in tissue regeneration. |
