Ginsenoside Rb1 alleviates lipopolysaccharide-induced inflammation in human dental pulp cells via the PI3K/Akt, NF-κB, and MAPK signalling pathways.

Autor: Nam OH; Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.; Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee Universtiy Medical Center, Seoul, Korea., Kim JH; Department of Pediatric Dentistry, School of Dentistry, Jeonbuk National University, Jeonju, Korea., Kang SW; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Chae YK; Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee Universtiy Medical Center, Seoul, Korea., Jih MK; Department of Pediatric Dentistry, School of Dentistry, Chosun University, Gwangju, Korea., You HH; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Koh JT; Department of Pharmacology and Dental Therapeutics, Hard-tissue Biointerface Research Center, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea., Kim Y; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea.
Jazyk: angličtina
Zdroj: International endodontic journal [Int Endod J] 2024 Jun; Vol. 57 (6), pp. 759-768. Date of Electronic Publication: 2024 Mar 04.
DOI: 10.1111/iej.14058
Abstrakt: Aim: Among numerous constituents of Panax ginseng, a constituent named Ginsenoside Rb1 (G-Rb1) has been studied to diminish inflammation associated with diseases. This study investigated the anti-inflammatory properties of G-Rb1 on human dental pulp cells (hDPCs) exposed to lipopolysaccharide (LPS) and aimed to determine the underlying molecular mechanisms.
Methodology: The KEGG pathway analysis was performed after RNA sequencing in G-Rb1- and LPS-treated hDPCs. Reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis were used for the assessment of cell adhesion molecules and inflammatory cytokines. Statistical analysis was performed with one-way ANOVA and the Student-Newman-Keuls test.
Results: G-Rb1 did not exhibit any cytotoxicity within the range of concentrations tested. However, it affected the levels of TNF-α, IL-6 and IL-8, as these showed reduced levels with exposure to LPS. Additionally, less mRNA and protein expressions of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were shown. With the presence of G-Rb1, decreased levels of PI3K/Akt, phosphorylated IκBα and p65 were also observed. Furthermore, phosphorylated ERK and JNK by LPS were diminished within 15, 30 and 60 min of G-Rb1 exposure; however, the expression of non-phosphorylated ERK and JNK remained unchanged.
Conclusions: G-Rb1 suppressed the LPS-induced increase of cell adhesion molecules and inflammatory cytokines, while also inhibiting PI3K/Akt, phosphorylation of NF-κB transcription factors, ERK and JNK of MAPK signalling in hDPCs.
(© 2024 British Endodontic Society. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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