Design and Evaluation of a Novel Anti-microbial Peptide from Cathelicidin-2: Selectively Active Against Acinetobacter baumannii .
| Autor: | Fathi F; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Ghobeh M; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., H Shirazi F; Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Tabarzad M; Protein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. |
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| Jazyk: | angličtina |
| Zdroj: | Iranian journal of pharmaceutical research : IJPR [Iran J Pharm Res] 2023 Dec 12; Vol. 22 (1), pp. e141920. Date of Electronic Publication: 2023 Dec 12 (Print Publication: 2023). |
| DOI: | 10.5812/ijpr-141920 |
| Abstrakt: | Background: Infections caused by pathogenic microorganisms have increased the need for hospital care and have thus represented a public health problem and a significant financial burden. Classical treatments consisting of traditional antibiotics face several challenges today. Anti-microbial peptides (AMPs) are a conserved characteristic of the innate immune response among different animal species to defend against pathogenic microorganisms. Objectives: In this study, a new peptide sequence (mCHTL131-140) was designed using the in silico approach. Methods: Cathelicidin-2 (UniprotID: Q2IAL7) was used as a potential antimicrobial protein, and a novel 10 - 12 amino acids sequence AMP was designed using bioinformatics tools and the AMP databases. Then, the anti-bacterial, anti-biofilm, and anti-fungal properties of the peptide, as well as its hemolytic activity and cytotoxicity towards human fibroblast (HDF) cells, were investigated in vitro. Results: Online bioinformatics tools indicated that the peptide sequence could have anti-bacterial, anti-viral, anti-fungal, and anti-biofilm properties with little hemolytic properties. The experimental tests confirmed that mCHTL131-140 exhibited the best anti-bacterial properties against Acinetobacter baumannii and had fair anti-fungal properties. Besides, it did not cause red blood cell lysis and showed no cytotoxicity towards HDF cells. Conclusions: In general, the designed peptide can be considered a promising AMP to control hospital-acquired infections by A. baumannii . Competing Interests: The authors declare that there is no conflict of interest. (Copyright © 2023, Fathi et al.) |
| Databáze: | MEDLINE |
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