Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection.

Autor:	Meshram S; Division of Plant Pathology, New Delhi 110 012, India., Gogoi R; Division of Plant Pathology, New Delhi 110 012, India., Bashyal BM; Division of Plant Pathology, New Delhi 110 012, India., Mandal PK; ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.; ICAR- National Institute for Plant Biotechnology, New Delhi 110 012, India., Hossain F; Division of Genetics, New Delhi 110 012, India., Kumar A; Division of Plant Pathology, New Delhi 110 012, India.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Feb 20; Vol. 10 (5), pp. e26538. Date of Electronic Publication: 2024 Feb 20 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e26538
Abstrakt:	Maydis leaf blight is a significant disease of maize caused by Bipolaris maydis race T, O and C . Molecular mechanisms regulating defense responses in non-CMS maize towards race O fungus are not fully known. In the present investigation, comparative transcriptome profiling was conducted on a highly resistant maize genotype SC-7-2-1-2-6-1 against a standard susceptible variety CM 119 at 48 h post inoculation (h PI) along with non-infected control. mRNA sequencing generated 38.4 Gb data, where 9349602 reads were mapped uniquely in SC-7, whereas 2714725 reads were mapped uniquely in CM-119. In inoculated SC-7, the total number of differentially expressed genes (DEGs) against control was 1413, where 1011 were up-regulated, and 402 were down-regulated. In susceptible inoculated genotype CM 119, the number of DEGs against control was 2902, where 1703 were up-, and 1199 were down-regulated. DEGs between inoculated resistant and susceptible genotypes were 10745, where 5343 were up-, and 5402 were down-regulated. The RNA-seq data were validated using RT-qPCR. The key findings are that SC-7 poses a robust plant signaling system mainly induced by oxidation-reduction process and calcium-mediated signaling. It regulates its fitness-related genes efficiently, viz., aldolase 2 gene, isopropanoid, phyto hormones, P450 cytochrome, amino acid synthesis, nitrogen assimilation genes etc. These findings showed more transcriptional changes in the SC-7 genotype, which contains many defence-related genes. They can be explored in future crop development programmes to combat multiple maize diseases. The current finding provides information to elucidate molecular and cellular processes occurring in maize during B. maydis race O infection. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as no potential competing interests: Shweta Meshram (First author) reports financial support was provided by NAHEP- CAAST project. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 Published by Elsevier Ltd.)
Databáze:	MEDLINE
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