Premixed calcium silicate-based ceramic sealers promote osteogenic/cementogenic differentiation of human periodontal ligament stem cells: A microscopy study.

Autor: López-García S; Departament d'Estomatologia, Facultat de Medicina I Odontologia, Universitat de València, Valencia, Spain., Sánchez-Bautista S; Department of Health Sciences, Catholic University San Antonio of Murcia, Murcia, Spain., García-Bernal D; Department of Biochemistry, Molecular Biology B and Immunology, Faculty of Medicine, University of Murcia, IMIB Pascual Parrilla, Murcia, Spain., Lozano A; Departament d'Estomatologia, Facultat de Medicina I Odontologia, Universitat de València, Valencia, Spain., Forner L; Departament d'Estomatologia, Facultat de Medicina I Odontologia, Universitat de València, Valencia, Spain., Sanz JL; Departament d'Estomatologia, Facultat de Medicina I Odontologia, Universitat de València, Valencia, Spain., Murcia L; Department of Health Sciences, Catholic University San Antonio of Murcia, Murcia, Spain., Rodríguez-Lozano FJ; Department of Dermatology, Stomatology, Radiology and Physical Medicine, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, IMIB Pascual Parrilla, Murcia, Spain., Oñate-Sánchez RE; Department of Dermatology, Stomatology, Radiology and Physical Medicine, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, IMIB Pascual Parrilla, Murcia, Spain.
Jazyk: angličtina
Zdroj: Microscopy research and technique [Microsc Res Tech] 2024 Jul; Vol. 87 (7), pp. 1584-1597. Date of Electronic Publication: 2024 Mar 03.
DOI: 10.1002/jemt.24545
Abstrakt: To evaluate the effects of premixed calcium silicate based ceramic sealers on the viability and osteogenic/cementogenic differentiation of human periodontal ligament stem cells (hPDLSCs). The materials evaluated were TotalFill BC Sealer (TFbc), AH Plus Bioceramic Sealer (AHPbc), and Neosealer Flo (Neo). Standardized discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following in vitro experiments were carried out: ion release, cell metabolic activity 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell migration, immunofluorescence experiment, cell attachment, gene expression, and mineralization assay. Statistical analyses were performed using one-way ANOVA followed by Tukey's post hoc test (p < .05). Increased Ca 2+ release was detected in TFbc compared to AHPbc and Neo (*p < .05). Biological assays showed a discrete cell metabolic activity and cell migration in Neo-treated cell, whereas scanning electronic microscopy assay exhibited that TFbc group had a better cell adhesion process of substrate attachment, spreading, and cytoskeleton development on the niche-like structures of the cement than AHPbc and Neo. The sealers tested were able to induce overexpression of the CEMP-1, ALP, and COL1A1 genes in the first days of exposure, particularly in the case of TFbc (***p < .001). All materials tested significantly increased the mineralization of hPDLSCs when compared to the negative control, although more pronounced calcium deposition was observed in the TFbc-treated cells (***p < .001). Our results suggested that TFbc promotes cell differentiation, both by increasing the expression of key osteo/odontogenic genes and by promoting mineralization of the extracellular matrix, whereas this phenomenon was less evident in Neo and AHPbc. RESEARCH HIGHLIGHTS: TFbc group had a better cell adhesion process of substrate attachment, spreading, and cytoskeleton development on the niche-like structures of the cement than AHPbc and Neo. The sealers tested were able to induce overexpression of the CEMP-1, ALP, and COL1A1 genes in the first days of exposure, particularly in the case of TFbc. All materials tested significantly increased the mineralization of hPDLSCs when compared to the negative control, although more pronounced calcium deposition was observed in the TFbc-treated cells.
(© 2024 The Authors. Microscopy Research and Technique published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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