| Autor: |
Tatsumi Y; Department of Operative Dentistry, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry., Kawaki H; Department of Oral Biochemistry, Division of Oral Structure, Function, and Development, Asahi University School of Dentistry., Shintani K; Department of Dental Materials Science, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry., Ueno K; Department of Oral Biochemistry, Division of Oral Structure, Function, and Development, Asahi University School of Dentistry., Hotta M; Asahi University., Kondoh N; Department of Chemistry, Division of Dental Basic Education, Asahi University School of Dentistry., Burrow MF; Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital., Nikaido T; Department of Operative Dentistry, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry. |
| Abstrakt: |
Surface pre-reacted glass-ionomer (S-PRG) filler is a bioactive glass filler capable of releasing various ions. A culture medium to which was added an S-PRG filler eluate rich in boron was reported to enhance alkaline phosphatase (ALP) activity in human dental pulp-derived stem cells (hDPSC). To clarify the role of boron eluted from S-PRG fillers, the modified S-PRG filler eluate with different boron concentrations was prepared by using an anion exchange material. Therefore, elemental mapping analysis of anion exchange material, adsorption ratio, hDPSCs proliferation and ALP activity were evaluated. For statistical analysis, Kruskal-Wallis test was used, with statistical significance determined at p<0.05. ALP activity enhancement was not observed in hDPSC cultured in the medium that contained the S-PRG filler eluate from which boron had been removed. The result suggested the possibility that an S-PRG filler eluate with controlled boron release could be useful for the development of novel dental materials. |
