The NADPH oxidase 2 subunit p47 phox binds to the WAVE regulatory complex and p22 phox in a mutually exclusive manner.
| Autor: | Kuihon SVNP; Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, Iowa, USA., Sevart BJ; Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, Iowa, USA., Abbey CA; Department of Medical Physiology, Texas A&M Health Science Center, Bryan, Texas, USA., Bayless KJ; Department of Medical Physiology, Texas A&M Health Science Center, Bryan, Texas, USA., Chen B; Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, Iowa, USA. Electronic address: stone@iastate.edu. |
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| Jazyk: | angličtina |
| Zdroj: | The Journal of biological chemistry [J Biol Chem] 2024 Apr; Vol. 300 (4), pp. 107130. Date of Electronic Publication: 2024 Mar 01. |
| DOI: | 10.1016/j.jbc.2024.107130 |
| Abstrakt: | The actin cytoskeleton and reactive oxygen species (ROS) both play crucial roles in various cellular processes. Previous research indicated a direct interaction between two key components of these systems: the WAVE1 subunit of the WAVE regulatory complex (WRC), which promotes actin polymerization and the p47 phox subunit of the NADPH oxidase 2 complex (NOX2), which produces ROS. Here, using carefully characterized recombinant proteins, we find that activated p47 phox uses its dual Src homology 3 domains to bind to multiple regions within the WAVE1 and Abi2 subunits of the WRC, without altering WRC's activity in promoting Arp2/3-mediated actin polymerization. Notably, contrary to previous findings, p47 phox uses the same binding pocket to interact with both the WRC and the p22 phox subunit of NOX2, albeit in a mutually exclusive manner. This observation suggests that when activated, p47 phox may separately participate in two distinct processes: assembling into NOX2 to promote ROS production and engaging with WRC to regulate the actin cytoskeleton. Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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