Polydopamine-based nano adjuvant as a promising vaccine carrier induces significant immune responses against Acinetobacter baumannii-associated pneumonia.

Autor: Sabzi S; Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran., Habibi M; Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran., Badmasti F; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Shahbazi S; Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran., Asadi Karam MR; Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: m_asadi@pasteur.ac.ir., Farokhi M; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran. Electronic address: m_farokhi@pasteur.ac.ir.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2024 Apr 10; Vol. 654, pp. 123961. Date of Electronic Publication: 2024 Mar 02.
DOI: 10.1016/j.ijpharm.2024.123961
Abstrakt: The objective of this study was to assess the effectiveness of polydopamine nanoparticles (PDANPs) as a delivery system for intranasal antigen administration to prevent Acinetobacter baumannii (A. baumannii)-associated pneumonia. In the in vitro phase, the conserved outer membrane protein 22 (Omp22)-encoding gene of A. baumannii was cloned, expressed, and purified, resulting in the production of recombinant Omp22 (rOmp22), which was verified using western blot. PDANPs were synthesized using dopamine monomers and loaded with rOmp22 through physical adsorption. The rOmp22-loaded PDANPs were characterized in terms of size, size distribution, zeta potential, field emission scanning electron microscopy (FESEM), loading capacity, Fourier transform infrared spectroscopy (FTIR), release profile, and cytotoxicity. In the in vivo phase, the adjuvant effect of rOmp22-loaded PDANPs was evaluated in terms of eliciting immune responses, including humoral and cytokine levels (IL-4, IL-17, and IFN-γ), as well as protection challenge. The rOmp22-loaded PDANPs were spherical with a size of 205 nm, a zeta potential of -14 mV, and a loading capacity of approximately 35.7 %. The released rOmp22 from nontoxic rOmp22-loaded PDANPs over 20 days was approximately 41.5 %, with preserved rOmp22 integrity. The IgG2a/IgG1 ratio and IFN-γ levels were significantly higher in immunized mice with rOmp22-loaded-PDANPs than in rOmp22-alum, naive Omp22, and control groups. Furthermore, rOmp22-loaded PDANPs induced effective protection against infection in the experimental challenge and showed more normal structures in the lung histopathology assay. The results of this study suggest the potential of PDANPs as a nano-adjuvant for inducing strong immune responses to combat A. baumannii.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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