Halicin remains active against Staphylococcus aureus in biofilms grown on orthopaedically relevant substrates.

Autor:	Higashihira S; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Department of Orthopaedic Surgery, Yokohama City University, Yokohama, Japan.; Department of Orthopaedic Surgery, Yokohama City University Medical Center, Yokohama, Japan., Simpson SJ; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, Indiana, USA., Morita A; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Department of Orthopaedic Surgery, Yokohama City University, Yokohama, Japan., Suryavanshi JR; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, Indiana, USA., Arnold CJ; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA., Natoli RM; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, Indiana, USA., Greenfield EM; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Jazyk:	angličtina
Zdroj:	Bone & joint research [Bone Joint Res] 2024 Mar 04; Vol. 13 (3), pp. 101-109. Date of Electronic Publication: 2024 Mar 04.
DOI:	10.1302/2046-3758.133.BJR-2023-0038.R2
Abstrakt:	Aims: Biofilm infections are among the most challenging complications in orthopaedics, as bacteria within the biofilms are protected from the host immune system and many antibiotics. Halicin exhibits broad-spectrum activity against many planktonic bacteria, and previous studies have demonstrated that halicin is also effective against Staphylococcus aureus biofilms grown on polystyrene or polypropylene substrates. However, the effectiveness of many antibiotics can be substantially altered depending on which orthopaedically relevant substrates the biofilms grow. This study, therefore, evaluated the activity of halicin against less mature and more mature S. aureus biofilms grown on titanium alloy, cobalt-chrome, ultra-high molecular weight polyethylene (UHMWPE), devitalized muscle, or devitalized bone. Methods: S. aureus -Xen36 biofilms were grown on the various substrates for 24 hours or seven days. Biofilms were incubated with various concentrations of halicin or vancomycin and then allowed to recover without antibiotics. Minimal biofilm eradication concentrations (MBECs) were defined by CFU counting and resazurin reduction assays, and were compared with the planktonic minimal inhibitory concentrations (MICs). Results: Halicin continued to exert significantly (p < 0.01) more antibacterial activity against biofilms grown on all tested orthopaedically relevant substrates than vancomycin, an antibiotic known to be affected by biofilm maturity. For example, halicin MBECs against both less mature and more mature biofilms were ten-fold to 40-fold higher than its MIC. In contrast, vancomycin MBECs against the less mature biofilms were 50-fold to 200-fold higher than its MIC, and 100-fold to 400-fold higher against the more mature biofilms. Conclusion: Halicin is a promising antibiotic that should be tested in animal models of orthopaedic infection. Competing Interests: E. M. Greenfield reports the Pilot and Feasibility Award within the CDMD NIH/NIDDK (Grant Number P30 DK097512), and materials (Co-Cr and highly crosslinked UHMWPE discs, gifted by Drs. Leonard Buller and Evan Deckard (IUSM Department of Orthopaedic Surgery) and muscle and bone tissue from New Zealand White rabbits gifted by Drs. Uma Sankar and Julian Dilley (IUSM Department of Anatomy, Cell Biology & Physiology), for the purpose of this study. S. Higashihira reports a Dokkyo Medical University Alumni Association Research Grant for this study. J. Suryavanshi reports the following institutional grants: Orthopaedic Research and Education Foundation (No. 22-068), Orthopaedic Trauma Association (No. 7950), and NIH Comprehensive Musculoskeletal Training Grant (T32), all for the purpose of this study. (© 2024 Higashihira et al.)
Databáze:	MEDLINE
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