Multiomics unravels potential molecular switches in the C 3 to CAM transition of Mesembryanthemum crystallinum.

Autor: Guan Q; Department of Biology, University of Mississippi, Oxford, MS 38677, USA., Kong W; College of Life Sciences, Northeast Agricultural University, Harbin 150040, China., Tan B; Department of Biology, University of Mississippi, Oxford, MS 38677, USA., Zhu W; Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Hangzhou 310002, China., Akter T; Department of Biology, University of Mississippi, Oxford, MS 38677, USA., Li J; College of Life Sciences, Northeast Agricultural University, Harbin 150040, China., Tian J; Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Hangzhou 310002, China., Chen S; Department of Biology, University of Mississippi, Oxford, MS 38677, USA. Electronic address: schen8@olemiss.edu.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2024 May 15; Vol. 299, pp. 105145. Date of Electronic Publication: 2024 Feb 29.
DOI: 10.1016/j.jprot.2024.105145
Abstrakt: Mesembryanthemum crystallinum (common ice plant), a facultative CAM plant, shifts from C 3 to CAM photosynthesis under salt stress, enhancing water use efficiency. Here we used transcriptomics, proteomics, and targeted metabolomics to profile molecular changes during the diel cycle of C 3 to CAM transition. The results confirmed expected changes associated with CAM photosynthesis, starch biosynthesis and degradation, and glycolysis/gluconeogenesis. Importantly, they yielded new discoveries: 1) Transcripts displayed greater circadian regulation than proteins. 2) Oxidative phosphorylation and inositol methylation may play important roles in initiating the transition. 3) V-type H + -ATPases showed consistent transcriptional regulation, aiding in vacuolar malate uptake. 4) A protein phosphatase 2C, a major component in the ABA signaling pathway, may trigger the C 3 to CAM transition. Our work highlights the potential molecular switches in the C 3 to CAM transition, including the potential role of ABA signaling. SIGNIFICANCE: The common ice plant is a model facultative CAM plant, and under stress conditions it can shift from C 3 to CAM photosynthesis within a three-day period. However, knowledge about the molecular changes during the transition and the molecular switches enabling the transition is lacking. Multi-omic analyses not only revealed the molecular changes during the transition, but also highlighted the importance of ABA signaling, inositol methylation, V-type H + -ATPase in initiating the shift. The findings may explain physiological changes and nocturnal stomatal opening, and inform future synthetic biology effort in improving crop water use efficiency and stress resilience.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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