Impact of L-Arginine on diabetes-induced neuropathy and myopathy: Roles of PAI-1, Irisin, oxidative stress, NF-κβ, autophagy and microRNA-29a.

Autor: Galal HM; Department of Medical Physiology, College of Medicine, Jouf University, Sakaka, Saudi Arabia; Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt., Abdelhafez AT; Department of Pharmacology, Faculty of Medicine, Assiut University, Assiut, Egypt; Department of Basic Medical Sciences, Badr University, New Nasser City, West of Assiut, Assiut, Egypt. Electronic address: alaa.abdelhafez@aun.edu.eg., Sayed MM; Histology and Cell Biology Department, Faculty of Medicine, Assiut University, Assiut, Egypt., Gomaa WMS; Department of Nutrition and Clinical Nutrition, Faculty of Vet. Medicine, Assiut University, Assiut, Egypt., Tohamy TA; Medical Research Center, Assiut University, Assiut, Egypt., Gomaa AMS; Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt., El-Metwally TH; Biochemistry Division, Department of Pathology, College of Medicine, Jouf University, Sakaka, Saudi Arabia; Medical Biochemistry Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2024 Apr; Vol. 87, pp. 102342. Date of Electronic Publication: 2024 Feb 28.
DOI: 10.1016/j.tice.2024.102342
Abstrakt: Background: T2DM is a chronic disorder with progressive neuromuscular alterations. L-arginine (ARG) is the most common semi-essential amino acid having several metabolic functions.
Aim: to investigate the impact of L-arginine in combating diabetic-induced neuromyopathy and its possible mechanisms.
Materials & Methods: 24 rats were divided into CON, CON+ARG, DC, DC+ARG. Behavioral tests, Body weight (BW), fasting blood glucose (FBG), insulin, total antioxidant capacity (TAC), malondialdehyde (MDA), plasminogen activator inhibitor-1 (PAI-1), and irisin were done. Creatine kinase-MM (CK-MM), interleukin 4 (IL-4), interleukin 6 (IL-6), TAC, MDA, expression of microRNA-29a mRNA & light chain 3 protein were determined in muscle. Histological and NF-κβ immunohistochemical expression in muscle and nerve were assessed.
Results: ARG supplementation to diabetic rats improved altered behavior, significantly increased BW, insulin, TAC, irisin and Il-4, decreased levels of glucose, microRNA-29a, NF-κβ and LC3 expression, PAI-1, CK-MM and restored the normal histological appearance.
Conclusions: ARG supplementation potently alleviated diabetic-induced neuromuscular alterations.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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