Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels.

Autor:	Peñuela-Sierra LM; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA; College of Veterinary Medicine, Universyty of Tolima, Ibagué, Tolima, 730006299, Colombia., Aragão-Neto VL; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA., Lozano-Cruz P; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA; College of Veterinary Medicine, Universyty of Tolima, Ibagué, Tolima, 730006299, Colombia., Mejia-Abaunza JN; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA; College of Veterinary Medicine, Universyty of Tolima, Ibagué, Tolima, 730006299, Colombia., Ali M; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA., Cabañas-Ojeda J; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA., Yang Y; Kemin (China) Technologies Co., Zhuhai, 519040 , China., Alfaro-Wisaquillo MC; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA., Quintana-Ospina GA; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA., Vasanthakumari BL; Kemin Industries, Des Moines, Iowa 50317, USA., Wealleans A; Kemin Industries, Des Moines, Iowa 50317, USA., Lao Y; Kemin Industries, Des Moines, Iowa 50317, USA., Oviedo-Rondón EO; Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA. Electronic address: edgar_oviedo@ncsu.edu.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 May; Vol. 103 (5), pp. 103546. Date of Electronic Publication: 2024 Feb 13.
DOI:	10.1016/j.psj.2024.103546
Abstrakt:	Low crude protein (CP) diets can reduce nitrogen (N) excretion and costs by increasing N utilization efficiency. Exogenous proteases may further improve protein digestibility in low CP diets. This study first evaluated in vitro the efficacy of a multiprotease on amino acid (AA) release from feedstuffs and broiler feed. Later, a broiler study evaluated the effect of feeding corn-soybean meal diets containing 3 CP levels (17, 19, and 21% CP) with supplementation on top of 0 or 2,400 U/kg multiprotease on chicken growth performance, total tract CP, and ileal AA digestibilities, and energy utilization. Ross 708 male chickens were placed in 42 cages and assigned to 6 treatments resulting from a 3 × 2 factorial arrangement. Three isocaloric basal diets were formulated to reduce CP, but all diets maintained digestible Lys:CP in 5.47% and the same ideal protein profile. Data were analyzed in a completely randomized design. On average, the multiprotease increased (P < 0.05) in vitro free AA release by 27.81% in most feedstuffs evaluated compared to the control. For broiler feed, 1,200 U/g multiprotease addition improved (P < 0.001) in vitro free AA release by 18.90%. This multiprotease showed interaction effects (P < 0.05) on chicken FCR, energy, and CP digestibility. As expected, BW at 24 d, BW gain, and FCR (8-24 d) worsened (P < 0.001) as dietary CP reduced from 21 to 17%, and multiprotease addition did not improve (P > 0.05) these parameters. BW gain decreased by 12.9% when N intake was reduced from 49.32 to 38.49 g/bird. Multiprotease supplementation improved (P < 0.01) AMEn by 71 kcal/kg, CP digestibility from 59.45 to 63.51%, ileal AA digestibility, and DM digestibility from 67.08 to 73.49%, but only in the 21% CP diet. No differences in ileal AA digestibility due to CP level (P > 0.05) were detected, except for Cys digestibility (P < 0.01). In conclusion, low CP diets reduced growth performance and improved N utilization but negatively affected energy utilization efficiency. Exogenous multiprotease supplementation improved AME, AMEn, protein, ileal AA, and DM digestibility in the 21% CP diet without significantly affecting growth performance. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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