Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells.

Autor: Klimovič Š; CEITEC, Masaryk University, Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic., Beckerová D; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; ICRC, St. Anne's University Hospital, Brno, Czech Republic., Věžník J; CEITEC, Masaryk University, Brno, Czech Republic; Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic., Kabanov D; CEITEC, Masaryk University, Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic., Lacina K; CEITEC, Masaryk University, Brno, Czech Republic., Jelinkova S; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Gumulec J; Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Rotrekl V; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; ICRC, St. Anne's University Hospital, Brno, Czech Republic., Přibyl J; CEITEC, Masaryk University, Brno, Czech Republic. Electronic address: jan.pribyl@ceitec.muni.cz.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2024 May; Vol. 159, pp. 213819. Date of Electronic Publication: 2024 Feb 28.
DOI: 10.1016/j.bioadv.2024.213819
Abstrakt: Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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