Bacillus subtilis-597 induces changes in lung pathology and inflammation during influenza A virus infection in pigs.

Autor: Winther K; Animal and Plant Health & Nutrition, Chr. Hansen A/S, Hoersholm, Denmark; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark., Kristensen C; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark., Henriksen BL; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs Lyngby, Denmark., Hansen LHB; Animal and Plant Health & Nutrition, Chr. Hansen A/S, Hoersholm, Denmark., Ryt-Hansen P; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark., Vestergaard G; Animal and Plant Health & Nutrition, Chr. Hansen A/S, Hoersholm, Denmark., Skovgaard K; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs Lyngby, Denmark., Sandvang D; Animal and Plant Health & Nutrition, Chr. Hansen A/S, Hoersholm, Denmark., Boll EJ; Animal and Plant Health & Nutrition, Chr. Hansen A/S, Hoersholm, Denmark., Williams AR; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark. Electronic address: arw@sund.ku.dk., Larsen LE; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Apr; Vol. 291, pp. 110032. Date of Electronic Publication: 2024 Feb 24.
DOI: 10.1016/j.vetmic.2024.110032
Abstrakt: In recent years, it has become apparent that imbalances in the gastrointestinal system can impact organs beyond the intestine such as the lungs. Given the established ability of probiotics to modulate the immune system by interacting with gastrointestinal cells, our research aimed to investigate whether administering the probiotic strain Bacillus subtilis-597 could mitigate the outcome of influenza virus infection in pigs. Pigs were fed a diet either with or without the probiotic strain B. subtilis-597 for 14 days before being intranasally inoculated with a swine influenza A H1N2 strain (1 C.2 lineage). Throughout the study, we collected fecal samples, blood samples, and nasal swabs to examine viral shedding and immune gene expression. After seven days of infection, the pigs were euthanized, and lung and ileum tissues were collected for gene expression analysis and pathological examination. Our findings indicate that the administration of B. subtilis-597 exhibit potential in reducing lung lesions, possibly attributable to a general suppression of the immune system as indicated by reduced C-reactive protein (CRP) levels in serum, decreased expression of interferon-stimulated genes (ISGs), and localized reduction of the inflammatory marker serum amyloid A (SAA) in ileum tissue. Notably, the immune-modulatory effects of B. subtilis-597 appeared to be unrelated to the gastrointestinal microbiota, as the composition remained unaltered by both the influenza infection and the administration of B. subtilis-597.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: KW, LHBH, GV, DS and EJB are employees of Chr Hansen A/S. ARW has received research funding from Chr Hansen A/S. The other authors declare no conflicts of interest.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: