Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus).

Autor: Jacott CN; Crop Genetics Department, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Schoonbeek HJ; Crop Genetics Department, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Sidhu GS; Computational and Systems Biology Department, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Steuernagel B; Computational and Systems Biology Department, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Kirby R; Crop Genetics Department, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Zheng X; Department of Crop Sciences, Georg August University, 37077, Göttingen, Germany., von Tiedermann A; Department of Crop Sciences, Georg August University, 37077, Göttingen, Germany., Macioszek VK; Department of Biology and Plant Ecology, Faculty of Biology, University of Bialystok, 15-245, Białystok, Poland., Kononowicz AK; Department of Plant Ecophysiology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237, Lodz, Poland., Fell H; Centre for Agriculture, Food and Environmental Management Research, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire, AL10 9AB, UK., Fitt BDL; Centre for Agriculture, Food and Environmental Management Research, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire, AL10 9AB, UK., Mitrousia GK; Centre for Agriculture, Food and Environmental Management Research, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire, AL10 9AB, UK.; Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK., Stotz HU; Centre for Agriculture, Food and Environmental Management Research, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire, AL10 9AB, UK., Ridout CJ; Crop Genetics Department, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Wells R; Crop Genetics Department, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. rachel.wells@jic.ac.uk.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Mar 02; Vol. 137 (3), pp. 65. Date of Electronic Publication: 2024 Mar 02.
DOI: 10.1007/s00122-024-04569-1
Abstrakt: Key Message: Using associative transcriptomics, our study identifies genes conferring resistance to four diverse fungal pathogens in crops, emphasizing key genetic determinants of multi-pathogen resistance. Crops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated with Brassica napus constitutive QDR to four contrasting fungal pathogens: Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae, and Verticillium longisporum. We did not identify any shared loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen-hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance to V. longisporum and potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight constitutive expressed candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. In conclusion, this study represents an advancement in our understanding of broad-spectrum QDR in B. napus and is a significant resource for the scientific community.
(© 2024. The Author(s).)
Databáze: MEDLINE
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