Rapid microdissection of tissue sections via laser ablation.
| Autor: | Coope RJ; Canada's Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada rcoope@bcgsc.ca., Pleasance S; Canada's Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada., Pandoh P; Canada's Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada., Schlosser C; Canada's Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada., Corbett RD; Canada's Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada., Marra MA; Canada's Michael Smith Genome Sciences Centre, Vancouver, British Columbia, Canada.; Medical Genetics, The University of British Columbia, Vancouver, British Columbia, Canada.; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of clinical pathology [J Clin Pathol] 2024 May 17; Vol. 77 (6), pp. 430-434. Date of Electronic Publication: 2024 May 17. |
| DOI: | 10.1136/jcp-2023-209361 |
| Abstrakt: | We demonstrate a method for tissue microdissection using scanning laser ablation that is approximately two orders of magnitude faster than conventional laser capture microdissection. Our novel approach uses scanning laser optics and a slide coating under the tissue that can be excited by the laser to selectively eject regions of tissue for further processing. Tissue was dissected at 0.117 s/mm 2 without reduction in yield, sequencing insert size or base quality compared with undissected tissue. From eight cases, 58-416 mm 2 of tissue was obtained from one to four slides in 7-48 seconds total dissection time per case. These samples underwent exome sequencing and we found the variant allelic fraction increased in regions enriched for tumour as expected. This suggests that our ablation technique may be useful as a tool in both clinical and research labs. Competing Interests: Competing interests: None declared. (© Author(s) (or their employer(s)) 2024. No commercial re-use. See rights and permissions. Published by BMJ.) |
| Databáze: | MEDLINE |
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