Characterisation of experimental flowable composites containing fluoride-doped calcium phosphates as promising remineralising materials.

Autor: Alambiaga-Caravaca AM; Department of Pharmacy, Faculty of Health Sciences, Institute of Biomedical Sciences, Cardenal Herrera-CEU University, CEU Universities, Valencia, Spain; Department of Anatomy & Regenerative Medicine, Tissue Engineering Research Group, Royal College of Surgeons in Ireland, Dublin, Ireland., Chou YF; Department of Dentistry (Dental Biomaterials and Minimally Invasive Dentistry), Faculty of Health Sciences, Univeristy CEU Cardenal Herrera, CEU Universities, Alfara del Patriarca, Valencia 46115, Spain., Moreno D; Division of Research, Faculty of Odontology, UIC Barcelona - Universitat Internacional de Catalunya, Sant Cugat del Vallès, Barcelona, Spain., Aparicio C; Division of Research, Faculty of Odontology, UIC Barcelona - Universitat Internacional de Catalunya, Sant Cugat del Vallès, Barcelona, Spain; IBEC-Institute for Bioengineering of Catalonia, Barcelona, Spain., López-Castellano A; Department of Pharmacy, Faculty of Health Sciences, Institute of Biomedical Sciences, Cardenal Herrera-CEU University, CEU Universities, Valencia, Spain., Feitosa VP; Department of Operative Dentistry, College of Dentistry, University of Iowa, Iowa City, USA., Tezvergil-Mutluay A; Adhesive Dentistry Research Group, Institute of Dentistry, and TYKS University Hospital, University of Turku, Turku, Finland., Sauro S; Department of Dentistry (Dental Biomaterials and Minimally Invasive Dentistry), Faculty of Health Sciences, Univeristy CEU Cardenal Herrera, CEU Universities, Alfara del Patriarca, Valencia 46115, Spain. Electronic address: salvatore.sauro@uch.ceu.es.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2024 Apr; Vol. 143, pp. 104906. Date of Electronic Publication: 2024 Feb 28.
DOI: 10.1016/j.jdent.2024.104906
Abstrakt: Objective: Remineralising composites with antibacterial properties may seal the cavity and prevent secondary caries. This study aimed at developing experimental flowable composites containing different concentrations of fluoride-doped calcium phosphate fillers and evaluating their remineralising and antibacterial properties.
Methods: Experimental resin-based composites containing different concentrations (0-20 %) of fluoride-doped calcium phosphate fillers (VS10/VS20) were formulated. The release of calcium (Ca), phosphate (PO) and fluoride (F) ions was assessed for 30 days. Remineralisation properties were evaluated through ATR-FTIR and SEM/EDX after storage in simulated body fluid (SBF). The metabolic activity and viability of Streptococcus gordonii was also evaluated through ATP, CFU and live/dead confocal microscopy. The evaluation of specific monomer elution from the experimental composites was conducted using high-performance liquid chromatography (HPLC).
Results: The composites containing VS10 showed the highest release of Ca, those containing VS20 released more F over time (p < 0.05), while there was no significant difference in terms of PO ions release between the groups (p > 0.05). A quick 7-day mineral precipitation was observed in the tested composites containing VS10 or VS20 at 10 %; these materials also showed the greatest antibacterial activity (p < 0.05). Moreover, the tested composites containing VS10 presented the lowest elution of monomers (p < 0.05).
Conclusions: Innovative composites were developed with low monomers elution, evident antibacterial activity against S. gordonii and important remineralisation properties due to specific ions release.
Clinical Significance: Novel composites containing fluoride-doped calcium phosphates may be promising to modulate bacteria growth, promote remineralisation and reduce the risk of cytotoxicity related to monomers' elution.
Competing Interests: Declaration of competing interest None of the authors have any conflict of interest with this study.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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