Bioactive C-phycocyanin exerts immunomodulatory and antitumor activity in mice with induced melanoma.

Autor: Salgado MTSF; Programa de Pós-Graduação em Ciências Fisiológicas, ICB, Universidade Federal do Rio Grande, FURG, Rio Grande, RS, Brazil; Laboratório de Cultura Celular, ICB, FURG, Rio Grande, RS, Brazil. Electronic address: marianasantossalgado@yahoo.com.br., Silva MCS; Laboratório de Imunorregulação, Departamento de Imunobiologia, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brazil; Programa de Pós-Graduação em Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brazil., Fratelli C; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Vila Mathias, Santos, SP, Brazil., Braga ARC; Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Vila Mathias, Santos, SP, Brazil., Lopes TBG; Laboratório do Comportamento Celular, ICB, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Ferreira E; Laboratório do Comportamento Celular, ICB, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., da Silva ILD; Programa de Pós-Graduação em Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brazil; Departamento de Patologia, Universidade Federal Fluminense, Niterói, RJ, Brazil., Paiva LS; Laboratório de Imunorregulação, Departamento de Imunobiologia, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brazil; Programa de Pós-Graduação em Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brazil., Votto APS; Programa de Pós-Graduação em Ciências Fisiológicas, ICB, Universidade Federal do Rio Grande, FURG, Rio Grande, RS, Brazil; Laboratório de Cultura Celular, ICB, FURG, Rio Grande, RS, Brazil.
Jazyk: angličtina
Zdroj: Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2024 Mar; Vol. 484, pp. 116874. Date of Electronic Publication: 2024 Feb 28.
DOI: 10.1016/j.taap.2024.116874
Abstrakt: Melanoma is the most aggressive and deadly skin cancer. The difficulty in its treatment arises from its ability to suppress the immune system, making it crucial to find a substance that increases anti-tumor immunity. C-phycocyanin (C-PC) appears as a promising bioactive, with multifaceted effects against several cancers, but its efficacy against melanoma has only been tested in vitro. Therefore, we investigated C-PC's the anti-tumor and immunomodulatory action in a murine melanoma model. The tumor was subcutaneously induced in C57BL/6 mice by injecting B16F10 cells. The animals were injected subcutaneously with C-PC for three consecutive days. After euthanasia, the tumor was weighed and measured. The inguinal lymph node was removed, and the cells were stained with antibodies and analyzed by flow cytometry. The heart, brain and lung were analyzed by histopathology. C-PC increased the B cell population of the inguinal lymph node in percentage and absolute number. The absolute number of T lymphocytes and myeloid cells were also increased in the groups treated with C-PC. Thus, C-PC showed a positive immunomodulatory effect both animals with and without tumor. However, this effect was more pronounced in the presence of the tumor. Positive immune system modulation may be associated with a reduction in tumor growth in animals treated with C-PC. Administration of C-PC subcutaneously did not cause organ damage. Our findings demonstrate C-PC's immunomodulatory and anti-melanoma action, paving the way for clinical research with this bioactive.
Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have influenced the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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