ROS Detection and Quantification in Plant-Nematode Interactions.

Autor: De Kock K; Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium., Matthys J; Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium., Kyndt T; Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. tina.kyndt@ugent.be.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2756, pp. 305-316.
DOI: 10.1007/978-1-0716-3638-1_10
Abstrakt: Reactive oxygen species (ROS) accumulation is one of the earliest hallmarks upon successful pathogen recognition in plants. H 2 O 2 is considered the most important ROS in plant defense considering its relatively high stability and capacity to cross long distances in the plant. However, ROS also play roles in cell development and could hence facilitate nematode feeding site development. Several methods to analyze the cellular redox state exist, among which ROS detection and quantification and the evaluation of ROS scavenging enzyme activity (peroxidase activity, catalase activity, etc.). Here, we describe DAB staining, which is used to detect and localize ROS in planta upon an external trigger. Furthermore, ROS quantification using the FOX assay is described. Both methods have been used extensively in research and yield repeatable results in various plants.
(© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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