Quantification of Neuronal Dendritic Spine Density and Lengths of Apical and Basal Dendrites in Temporal Lobe Structures Using Golgi-Cox Staining.
| Autor: | Dubey V; Department of Biophysics, AIIMS, New Delhi, India., Dixit AB; Dr B R Ambedkar Centre for Biomedical Research, University of Delhi, Delhi, India., Tripathi M; Department of Neurology, AIIMS, New Delhi, India., Sarat Chandra P; Department of Neurosurgery, AIIMS, New Delhi, India., Banerjee J; Department of Biophysics, AIIMS, New Delhi, India. jyotirmoy@aiims.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2761, pp. 57-66. |
| DOI: | 10.1007/978-1-0716-3662-6_5 |
| Abstrakt: | The objective of this chapter is to provide an overview of the methods used to investigate the connectivity and structure of the nervous system. These methods allow neuronal cells to be categorized according to their location, shape, and connections to other cells. The Golgi-Cox staining gives a thorough picture of all significant neuronal structures found in the brain that may be distinguished from one another. The most significant characteristic is its three-dimensional integrity since all neuronal structures may be followed continuously from one part to the next. Successions of sections of the brain's neurons are seen with the Golgi stain. The Golgi method is used to serially segment chosen brain parts, and the resulting neurons are produced from those sections. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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