Serum starvation is as efficient as roscovitine on the cycle synchronization in G 0 /G 1 of red-rumped agouti fibroblasts.

Autor: Praxedes ÉA; Laboratory of Animal Biotechnology, Universidade Federal Rural Do Semi-Árido, Av. Francisco Mota, 572, Mossoró, RN, 59625-900, Brazil., Oliveira LRM; Laboratory of Animal Biotechnology, Universidade Federal Rural Do Semi-Árido, Av. Francisco Mota, 572, Mossoró, RN, 59625-900, Brazil., da Silva Viana JV; Laboratory of Animal Biotechnology, Universidade Federal Rural Do Semi-Árido, Av. Francisco Mota, 572, Mossoró, RN, 59625-900, Brazil., Rodrigues LLV; Laboratory of Animal Biotechnology, Universidade Federal Rural Do Semi-Árido, Av. Francisco Mota, 572, Mossoró, RN, 59625-900, Brazil., de Brito Vieira Neto J; Experimental Oncology Laboratory, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., Sales SLA; Experimental Oncology Laboratory, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., Dos Santos Luciano MC; Experimental Oncology Laboratory, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., Oliveira MF; Laboratory of Animal Biotechnology, Universidade Federal Rural Do Semi-Árido, Av. Francisco Mota, 572, Mossoró, RN, 59625-900, Brazil., Pessoa C; Experimental Oncology Laboratory, Universidade Federal Do Ceará, Fortaleza, CE, Brazil., Pereira AF; Laboratory of Animal Biotechnology, Universidade Federal Rural Do Semi-Árido, Av. Francisco Mota, 572, Mossoró, RN, 59625-900, Brazil. alexsandra.pereira@ufersa.edu.br.
Jazyk: angličtina
Zdroj: In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2024 Mar; Vol. 60 (3), pp. 249-257. Date of Electronic Publication: 2024 Mar 01.
DOI: 10.1007/s11626-024-00866-7
Abstrakt: Fibroblast cycle synchronization in G 0 /G 1 is an essential step for nuclear reprogramming by cloning or induced cells to pluripotency. Considering the diversity among rodents and the ecological and scientific importance of these animals, we compared the contact inhibition, serum starvation, and 10 µM of roscovitine as methods of synchronization of red-rumped agouti fibroblasts. The effects of each protocol were evaluated on the percentage of cycle phase, morphology, viability, and apoptosis levels. The results showed that culturing the cells to serum starvation for 24 h (75.9%), 48 h (81.6%), 72 h (86.2%), 96 h (84.0%), and 120 h (83.7%) yielded a significantly higher percentage of cells arrested in the G 0 /G 1 (P < 0.05) phase than cells not subjected to any cell cycle synchronization method (31.4%). Also, this effect was not different between the times of 48 and 120 h (P > 0.05). A similar response was observed for cells cultured with roscovitine for 12 h (86.9%), 24 h (74.8%), and 48 h (81.7%), with a higher percentage of synchronized cells in G 0 /G 1 compared to cells not submitted to any synchronization treatment (52.2%). Nevertheless, this effect was best evidenced at 12 h (P < 0.05). Also, the contact inhibition for 24-120 h could not synchronize cells in G 0 /G 1 , with values ranging from 70.9 to 77.9% (P > 0.05). Moreover, no difference was observed for morphology, viability, and apoptosis levels in any synchronization method (P > 0.05). Therefore, serum starvation is as efficient as roscovitine on cycle synchronization in G 0 /G 1 of red-rumped agouti fibroblasts.
(© 2024. The Society for In Vitro Biology.)
Databáze: MEDLINE
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