Molecular Prediction and Correlation of the Structure and Function of Universal Stress Protein A (UspA) from Salmonella Typhimurium.

Autor: Nabi B; Department of Biochemistry & Biochemical Engineering, SHUATS, Prayagraj, 211007, India., Kumawat M; Department of Microbiology, ICMR- National Institute for Research in Environmental Health, Bhopal, 462030, India., Yadav PK; Department of Computational Biology & Bioinformatics, SHUATS, Prayagraj, 211007, India., Ahlawat N; Department of Animal Husbandry and Dairying, SHUATS, Prayagraj, 211007, India., Mir MA; Department of Bioresources, School of Biological Sciences, University of Kashmir, Srinagar, 190006, India., Kumar V; Department of Computational Biology & Bioinformatics, SHUATS, Prayagraj, 211007, India., Kumar M; Department of Microbiology, ICMR- National Institute for Research in Environmental Health, Bhopal, 462030, India. manoj15micro@yahoo.co.in., Ahlawat S; Department of Biochemistry & Biochemical Engineering, SHUATS, Prayagraj, 211007, India. sahlawat64@gmail.com.
Jazyk: angličtina
Zdroj: Biochemical genetics [Biochem Genet] 2024 Mar 01. Date of Electronic Publication: 2024 Mar 01.
DOI: 10.1007/s10528-024-10699-4
Abstrakt: Salmonella Typhimurium (ST) is a zoonotic pathogen that can cause gastroenteritis in humans when they consume contaminated food or water. When exposed to various stressors, both from living organisms (biotic) and the environment (abiotic), Salmonella Typhimurium produces Universal Stress Proteins (USPs). These proteins are gaining recognition for their crucial role in bacterial stress resistance and the ability to enter a prolonged state of growth arrest. Additionally, USPs exhibit diverse structures due to the fusion of the USP domain with different catalytic motifs, enabling them to participate in various reactions and cellular activities during stressful conditions. In this particular study, researchers cloned and analyzed the uspA gene obtained from poultry-derived strains of Salmonella Typhimurium. The gene comprises 435 base pairs, encoding a USP family protein consisting of 144 amino acids. Phylogenetic analysis demonstrated a close relationship between the uspA genes of Salmonella Typhimurium and those found in other bacterial species. We used molecular dynamics simulations and 3D structure prediction to ensure that the USPA protein was stable. Furthermore, we also carried out motif search and network analysis of protein-protein interactions. The findings from this study offer valuable insights for the development of inhibitors targeted against Salmonella Typhimurium.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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