Perineuronal net in the extrinsic innervation of the distal colon of mice and its remodeling in ulcerative colitis.

Autor: da Silva MDV; Laboratory of Neurogastroenterology, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil.; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., da Silva Bonassa L; Laboratory of Neurogastroenterology, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Piva M; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., Basso CR; Laboratory of Neurogastroenterology, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Zaninelli TH; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., Machado CCA; Laboratory of Neurogastroenterology, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., de Andrade FG; Laboratory of Histopathological Analysis, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Miqueloto CA; Laboratory of Neurogastroenterology, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Sant Ana DMG; Enteric Neuroscience Laboratory, Department of Morphological Sciences, State University of Maringá, Maringá, Paraná, Brazil., Aktar R; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., Peiris M; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., Aziz Q; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., Blackshaw LA; Wingate Institute for Neurogastroenterology, Queen Mary University of London, London, UK., Verri WA; Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., de Almeida Araújo EJ; Laboratory of Neurogastroenterology, Department of Histology, State University of Londrina, Londrina, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Journal of neurochemistry [J Neurochem] 2024 Sep; Vol. 168 (9), pp. 1937-1955. Date of Electronic Publication: 2024 Mar 01.
DOI: 10.1111/jnc.16080
Abstrakt: The perineuronal net (PNN) is a well-described highly specialized extracellular matrix structure found in the central nervous system. Thus far, no reports of its presence or connection to pathological processes have been described in the peripheral nervous system. Our study demonstrates the presence of a PNN in the spinal afferent innervation of the distal colon of mice and characterizes structural and morphological alterations induced in an ulcerative colitis (UC) model. C57Bl/6 mice were given 3% dextran sulfate sodium (DSS) to induce acute or chronic UC. L6/S1 dorsal root ganglia (DRG) were collected. PNNs were labeled using fluorescein-conjugated Wisteria Floribunda (WFA) l lectin, and calcitonin gene-related peptide (CGRP) immunofluorescence was used to detect DRG neurons. Most DRG cell bodies and their extensions toward peripheral nerves were found surrounded by the PNN-like structure (WFA+), labeling neurons' cytoplasm and the pericellular surfaces. The amount of WFA+ neuronal cell bodies was increased in both acute and chronic UC, and the PNN-like structure around cell bodies was thicker in UC groups. In conclusion, a PNN-like structure around DRG neuronal cell bodies was described and found modulated by UC, as changes in quantity, morphology, and expression profile of the PNN were detected, suggesting a potential role in sensory neuron peripheral sensitization, possibly modulating the pain profile of ulcerative colitis.
(© 2024 International Society for Neurochemistry.)
Databáze: MEDLINE
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