Antioxidant polysaccharide-enriched fractions obtained from olive leaves by ultrasound-assisted extraction with α-amylase inhibition, and antiproliferative activities.

Autor: Elayeb R; Bioresources, Integrative Biology and Valorization Research Laboratory 'BIOLIVAL' (UR03ES09), Higher Institute of Biotechnology of Monastir, Monastir, Tunisia.; Department of Food Phytochemistry, Fat Institute (Spanish National Research Council, CSIC), Seville, Spain., Bermúdez-Oria A; Department of Food Phytochemistry, Fat Institute (Spanish National Research Council, CSIC), Seville, Spain., Lazreg Aref H; Laboratory of Genetics, Biodiversity and Bioresources Valuation LR11S41, Higher Institute of Biotechnology of Monastir, Monastir, Tunisia., Majdoub H; Laboratory of Interfaces and Advanced Materials, Faculty of Sciences of Monastir, University of Monastir, 5000 Monastir, Tunisia., Ritzoulis C; Department of Food Technology, ATEI of Thessaloniki, 57400 Thessaloniki, Greece., Mannu A; Department of Chemistry and Pharmacy, University of Sassari, Via Vienna 2, 07100 Sassari, Italy., Le Cerf D; UNIROUEN, INSA Rouen, CNRS, PBS, Normandie University, 76000 Rouen, France., Carraro M; Department of Chemistry and Pharmacy, University of Sassari, Via Vienna 2, 07100 Sassari, Italy., Achour S; Bioresources, Integrative Biology and Valorization Research Laboratory 'BIOLIVAL' (UR03ES09), Higher Institute of Biotechnology of Monastir, Monastir, Tunisia., Fernández-Bolaños J; Department of Food Phytochemistry, Fat Institute (Spanish National Research Council, CSIC), Seville, Spain., Trigui M; Bioresources, Integrative Biology and Valorization Research Laboratory 'BIOLIVAL' (UR03ES09), Higher Institute of Biotechnology of Monastir, Monastir, Tunisia.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2024 Mar; Vol. 14 (3), pp. 92. Date of Electronic Publication: 2024 Feb 27.
DOI: 10.1007/s13205-024-03939-2
Abstrakt: Polysaccharide-rich materials were extracted from the alcohol-insoluble solids of Olea europaea l. **leaves. Structural characteristics were determined by colorimetric techniques, FT-IR, GC-MS, SEC/MALS/VD/DRI, and NMR ( 1 H, 13 C). The extract and its main macromolecular components were characterized to assess their ability toward antioxidant, α-amylase inhibition, and antiproliferative activities. Results revealed that the ultrasound olive leave extract comprises polysaccharides with uronic acid, galactose, arabinose, and glucose in molar percentages of 11.7%, 11.3%, 7.5%, and 4.9% respectively, constituting 41% of the total mass. In addition, polyphenols (21%) and proteins (9%) are associated with these polysaccharides. Further, the extract showed noticeable ORAC and free radical scavenging abilities, in addition to high in vitro antiproliferative activity against Caco-2 colon carcinoma cell lines. Similarly, the extract exhibited a strong, uncompetitive inhibition of α-amylase by 75% in the presence of the extract with 0.75 µg/mL of concentration. This research concludes that ultrasound extraction method can be used for the extraction of polysaccharide-polyphenol-protein complexes. These conjugates exhibit the potential for combined biological activities resulting from a synergistic effect of its compounds, making them promising ingredients for the development of functional food.
Competing Interests: Conflict of interestThe authors solemnly vow that there are no competing financial interests in this work.
(© King Abdulaziz City for Science and Technology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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