Purification, biochemical characterization, and biotechnological applications of a multifunctional enzyme from the Thermoascus aurantiacus PI3S3 strain.

Autor: Battisti JA; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., Rocha GB; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., Rasbold LM; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., Delai VM; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., Costa MSSM; Centro de Ciências Exatas e Tecnológicas, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, 85819-110, Brazil., Kadowaki MK; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., da Conceição Silva JL; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., Simão RCG; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., Bifano TD; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil., Maller A; Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069 Universitária Street, Faculdade, Cascavel, Paraná, 85819-110, Brazil. alexandre.maller@unioeste.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Feb 29; Vol. 14 (1), pp. 5037. Date of Electronic Publication: 2024 Feb 29.
DOI: 10.1038/s41598-024-55665-7
Abstrakt: The filamentous Thermoascus aurantiacus fungus characterized by its thermophilic nature, is recognized as an exceptional producer of various enzymes with biotechnological applications. This study aimed to explore biotechnological applications using polygalacturonase (PG) derived from the Thermoascus aurantiacus PI3S3 strain. PG production was achieved through submerged fermentation and subsequent purification via ion-exchange chromatography and gel filtration methods. The crude extract exhibited a diverse spectrum of enzymatic activities including amylase, cellulase, invertase, pectinase, and xylanase. Notably, it demonstrated the ability to hydrolyze sugarcane bagasse biomass, corn residue, and animal feed. The purified PG had a molecular mass of 36 kDa, with optimal activity observed at pH 4.5 and 70 °C. The activation energy (Ea) was calculated as 0.513 kJ mol -1 , highlighting activation in the presence of Ca 2+ . Additionally, it displayed apparent K m , V max , and K cat values of at 0.19 mg mL -1 , 273.10 U mL -1 , and 168.52 s -1 , respectively, for hydrolyzing polygalacturonic acid. This multifunctional PG exhibited activities such as denim biopolishing, apple juice clarification, and demonstrated both endo- and exo-polygalacturonase activities. Furthermore, it displayed versatility by hydrolyzing polygalacturonic acid, carboxymethylcellulose, and xylan. The T. aurantiacus PI3S3 multifunctional polygalacturonase showed heightened activity under acidic pH, elevated temperatures, and in the presence of calcium. Its multifunctional nature distinguished it from other PGs, significantly expanding its potential for diverse biotechnological applications.
(© 2024. The Author(s).)
Databáze: MEDLINE
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