Two distinct subpopulations of marginal zone B cells exhibit differential antibody-producing capacities and radioresistance.

Autor: Lee S; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea., Ko Y; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea.; Immunology and Microbiology Graduate Program, Baylor College of Medicine, Houston, TX, 77030, USA., Lee HW; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea., Oh WJ; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea., Hong HG; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea., Ariyaratne D; Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka., Im SJ; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea. sejinim@skku.edu., Kim TJ; Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea. tjkim@skku.edu.
Jazyk: angličtina
Zdroj: Cellular & molecular immunology [Cell Mol Immunol] 2024 Apr; Vol. 21 (4), pp. 393-408. Date of Electronic Publication: 2024 Mar 01.
DOI: 10.1038/s41423-024-01126-0
Abstrakt: Marginal zone (MZ) B cells, which are splenic innate-like B cells that rapidly secrete antibodies (Abs) against blood-borne pathogens, are composed of heterogeneous subpopulations. Here, we showed that MZ B cells can be divided into two distinct subpopulations according to their CD80 expression levels. CD80 high MZ B cells exhibited greater Ab-producing, proliferative, and IL-10-secreting capacities than did CD80 low MZ B cells. Notably, CD80 high MZ B cells survived 2-Gy whole-body irradiation, whereas CD80 low MZ B cells were depleted by irradiation and then repleted with one month after irradiation. Depletion of CD80 low MZ B cells led to accelerated development of type II collagen (CII)-induced arthritis upon immunization with bovine CII. CD80 high MZ B cells exhibited higher expression of genes involved in proliferation, plasma cell differentiation, and the antioxidant response. CD80 high MZ B cells expressed more autoreactive B cell receptors (BCRs) that recognized double-stranded DNA or CII, expressed more immunoglobulin heavy chain sequences with shorter complementarity-determining region 3 sequences, and included more clonotypes with no N-nucleotides or with B-1a BCR sequences than CD80 low MZ B cells. Adoptive transfer experiments showed that CD21 + CD23 + transitional 2 MZ precursors preferentially generated CD80 low MZ B cells and that a proportion of CD80 low MZ B cells were converted into CD80 high MZ B cells; in contrast, CD80 high MZ B cells stably remained CD80 high MZ B cells. In summary, MZ B cells can be divided into two subpopulations according to their CD80 expression levels, Ab-producing capacity, radioresistance, and autoreactivity, and these findings may suggest a hierarchical composition of MZ B cells with differential stability and BCR specificity.
(© 2024. The Author(s), under exclusive licence to CSI and USTC.)
Databáze: MEDLINE
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