Generation of Fel d 1 chain 2 genome-edited cats by CRISPR-Cas9 system.

Autor: Lee SR; Animal, Dairy, and Veterinary Sciences Department, College of Agriculture and Applied Sciences, Utah State University, Logan, UT, 84322, USA.; Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA., Lee KL; TheKingKong Corp. Ltd., Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Song SH; TheKingKong Corp. Ltd., Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Joo MD; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Lee SH; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Kang JS; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Kang SM; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Idrees M; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Kim JW; TheKingKong Corp. Ltd., Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea., Kong IK; TheKingKong Corp. Ltd., Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea. ikong@gnu.ac.kr.; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea. ikong@gnu.ac.kr.; Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam Province, 52828, Republic of Korea. ikong@gnu.ac.kr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Feb 29; Vol. 14 (1), pp. 4987. Date of Electronic Publication: 2024 Feb 29.
DOI: 10.1038/s41598-024-55464-0
Abstrakt: Allergens from domestic cats (Felis catus) cause allergy-related health problems worldwide. Fel d 1 is a major allergen that causes severe allergic reactions in humans, including rhinitis, conjunctivitis, and life-threatening asthma. Therefore, patients with cat allergies anticipate hypoallergenic cats. We successfully generated Fel d 1 chain 2 (CH2) genome-edited cats using the CRISPR-Cas9 system in this study. T7 endonuclease 1 assay and Sanger sequencing were used to confirm the mutation in CH2 genome-edited cats. Fel d 1 level in CH2 genome-edited cats were assessed by enzyme-linked immunosorbent assay (ELISA). Remarkably, ELISA showed that the level of Fel d 1 in the CH2 homozygous genome-edited cat (Name: Alsik) was extremely low compared with that in wild type domestic cats and could be hypoallergenic cats. Additionally, we successfully cloned the CH2 homozygous genome-edited cat using cytoplasm injection clone technology. The cloned CH2 homozygous genome-edited cat was verified using microsatellite analysis. Creating hypoallergenic cats using the CRISPR-Cas9 system is a significant step forward because these cats can safely approach allergic patients.
(© 2024. The Author(s).)
Databáze: MEDLINE
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