Nitrogen-doped carbon quantum dots as a novel treatment for black fungal bone infections (Mucormycosis): in vitro and in vivo study.

Autor: Belal A; Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi Arabia., Almalki AH; Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi Arabia.; Addiction and Neuroscience Research Unit, College of Pharmacy, Taif University, Taif, Saudi Arabia., Farghali AA; Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Science (PSAS), Beni-Suef University, Beni-Suef, Egypt., Mahmoud R; Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Atta RR; Department of Chemistry, Faculty of Science, Damietta University, Damietta, Egypt., Allah AE; Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Hassan WH; Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt., Lee S; Department of Fundamental Pharmaceutical Science, Graduate School, Kyung Hee University, Seoul, Republic of Korea.; Department of Regulatory Science, Graduated School, Kyung Hee University, Seoul, Republic of Korea., Kotp AA; Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Science (PSAS), Beni-Suef University, Beni-Suef, Egypt., Essam D; Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Hassan AHE; Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.; Medicinal Chemistry Laboratory, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea., Ghoneim MM; Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Ad Diriyah, Saudi Arabia.; Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt., Abo El-Ela FI; Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt., Abdelwahab A; Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Science (PSAS), Beni-Suef University, Beni-Suef, Egypt.; Faculty of Science, Galala University, Sokhna, Egypt.
Jazyk: angličtina
Zdroj: Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2024 Dec; Vol. 52 (1), pp. 131-144. Date of Electronic Publication: 2024 Feb 29.
DOI: 10.1080/21691401.2024.2318212
Abstrakt: Most fungal bone and joint infections (arthritis) are caused by Mucormycosis ( Mucor indicus ). These infections may be difficult to treat and may lead to chronic bone disorders and disabilities, thus the use of new antifungal materials in bone disorders is vital, particularly in immunocompromised individuals, such as those who have contracted coronavirus disease 2019 (COVID-19). Herein, we reported for the first time the preparation of nitrogen-doped carbon quantum dots (N/CQDs) and a nitrogen-doped mesoporous carbon (N/MC) using a quick micro-wave preparation and hydrothermal approach. The structure and morphology were analysed using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and surface area analyser. Minimum inhibitory concentration (MIC), disc diffusion tests, minimum fungicidal concentration (MFC) and antifungal inhibitory percentages were measured to investigate the antifungal activity of N/CQDs and N/MC nanostructures. In addition to the in vivo antifungal activity in rats as determined by wound induction and infection, pathogen count and histological studies were also performed. According to in vitro and in vivo testing, both N/CQDs with small size and N/MC with porous structure had a significant antifungal impact on a variety of bone-infecting bacteria, including Mucor infection. In conclusion, the present investigation demonstrates that functional N/CQDs and N/MC are effective antifungal agents against a range of microbial pathogenic bone disorders in immunocompromised individuals, with stronger and superior fungicidal activity for N/CQDs than N/MC in vitro and in vivo studies.
Databáze: MEDLINE
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