Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol.

Autor: McCarthy CM; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.; School of Engineering, University of Limerick, Limerick, Ireland., McKevitt KL; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland., Connolly SA; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.; School of Engineering, University of Limerick, Limerick, Ireland., Andersson I; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.; School of Engineering, University of Limerick, Limerick, Ireland., Leahy FC; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland., Egan S; Department of Colorectal Surgery, University Hospital Limerick, Limerick, Ireland., Moloney MA; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland., Kavanagh EG; Department of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland., Peirce C; Department of Colorectal Surgery, University Hospital Limerick, Limerick, Ireland., Cunnane EM; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.; School of Engineering, University of Limerick, Limerick, Ireland.; Health Research Institute, University of Limerick, Limerick, Ireland., McGourty KD; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.; School of Engineering, University of Limerick, Limerick, Ireland.; School of Chemical Sciences, University of Limerick, Limerick, Ireland., Walsh MT; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.; School of Engineering, University of Limerick, Limerick, Ireland.; Health Research Institute, University of Limerick, Limerick, Ireland., Mulvihill JJE; Biomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.; School of Engineering, University of Limerick, Limerick, Ireland.; Health Research Institute, University of Limerick, Limerick, Ireland.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Feb 29; Vol. 19 (2), pp. e0297618. Date of Electronic Publication: 2024 Feb 29 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0297618
Abstrakt: Microindentation of fresh biological tissues is necessary for the creation of 3D biomimetic models that accurately represent the native extracellular matrix microenvironment. However, tissue must first be precisely sectioned into slices. Challenges exist in the preparation of fresh tissue slices, as they can tear easily and must be processed rapidly in order to mitigate tissue degradation. In this study, we propose an optimised mounting condition for microindentation and demonstrate that embedding tissue in a mixture of 2.5% agarose and 1.5% gelatin is the most favourable method of tissue slice mounting for microindentation. This protocol allows for rapid processing of fresh biological tissue and is applicable to a variety of tissue types.
Competing Interests: This research is funded by the Irish government through the Irish Research Council Enterprise Partnership Scheme in association with Becton Dickinson (EPSPG/2020/467). This does not alter our adherence to PLOS ONE policies on sharing data and materials.
(Copyright: © 2024 McCarthy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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