The ASPREE Healthy Ageing Biobank: Methodology and participant characteristics.

Autor: Parker EJ; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Orchard SG; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Gilbert TJ; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Phung JJ; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Owen AJ; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Lockett T; Health and Biosecurity, Commonwealth Scientific and Industrial Research Organisation, North Ryde, New South Wales, Australia.; Technical Director, Rhythm Biosciences Ltd, Parkville, Victoria, Australia., Nelson MR; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia.; Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania, Australia., Reid CM; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Tonkin AM; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Abhayaratna WP; ANU Medical School, Australian National University, Garran, Australian Capital Territory, Australia., Gibbs P; Department of Medicine, The University of Melbourne, Melbourne, Victoria, Australia., McNeil JJ; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia., Woods RL; School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Feb 29; Vol. 19 (2), pp. e0294743. Date of Electronic Publication: 2024 Feb 29 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0294743
Abstrakt: ASPirin in Reducing Events in the Elderly (ASPREE), a placebo-controlled prevention trial of low dose aspirin, provided the opportunity to establish a biospecimen biobank from initially healthy persons aged 70+ years for future research. The ASPREE Healthy Ageing Biobank (ASPREE Biobank) collected, processed and stored blood and urine samples at -80degC or under nitrogen vapour at two timepoints, three years apart, from a willing subset of Australian ASPREE participants. Written informed consent included separate opt-in questions for biomarker and genetic testing. Fractionated blood and urine were aliquoted into multiple low-volume, barcoded cryotubes for frozen storage within 4 hours of collection. Specially designed and outfitted mobile laboratories provided opportunities for participation by people in regional and rural areas. Detailed, high quality demographic, physiological and clinical data were collected annually through the ASPREE trial. 12,219 participants contributed blood/urine at the first timepoint, 10,617 of these older adults provided 3-year follow-up samples, and an additional 1,712 provided saliva for DNA. The mean participant age was 74 years, 54% were female and 46% lived outside major cities. Despite geographical and logistical challenges, nearly 100% of blood/urine specimens were processed and frozen within 4 hours of collection into >1.4 million aliquots. After a median of 4.7 years, major clinical events among ASPREE Biobank participants included 332 with dementia, 613 with cardiovascular disease events, 1259 with cancer, 357 with major bleeds and 615 had died. The ASPREE Biobank houses and curates a large number of biospecimens collected prior to the clinical manifestations of major disease, and 3-year follow-up samples, all linked to high quality, extensive phenotypic information. This provides the opportunity to identify or validate diagnostic, prognostic and predictive biomarkers, and potentially study biological effectors, of ageing-related diseases or maintenance of older-age good health.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Parker et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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