Functional analysis and transcriptome profile of meninges and skin fibroblasts from human-aged donors.

Autor: Fantini V; Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, Abbiategrasso, Italy., Ferrari RR; Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, Abbiategrasso, Italy., Bordoni M; Cellular Model and Neuroepigenetics Unit, IRCCS Mondino Foundation, Pavia, Italy., Spampinato E; Cellular Model and Neuroepigenetics Unit, IRCCS Mondino Foundation, Pavia, Italy.; Department of Biology and Biotechnology, University of Pavia, Pavia, Italy., Pandini C; Molecular Biology and Transcriptomics Unit, IRCCS Mondino Foundation, Pavia, Italy.; Department of Biosciences, University of Milan, Milan, Italy., Davin A; Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, Abbiategrasso, Italy., Medici V; Department of Neurology and Neuropathology, Golgi-Cenci Foundation, Abbiategrasso, Italy., Gagliardi S; Molecular Biology and Transcriptomics Unit, IRCCS Mondino Foundation, Pavia, Italy., Guaita A; Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, Abbiategrasso, Italy.; Department of Neurology and Neuropathology, Golgi-Cenci Foundation, Abbiategrasso, Italy., Pansarasa O; Cellular Model and Neuroepigenetics Unit, IRCCS Mondino Foundation, Pavia, Italy., Cereda C; Center of Functional Genomics and Rare Diseases, Department of Pediatrics, Buzzi Children's Hospital, Milan, Italy., Poloni TE; Department of Neurology and Neuropathology, Golgi-Cenci Foundation, Abbiategrasso, Italy.; Department of Rehabilitation, ASP Golgi-Redaelli Geriatric Hospital, Abbiategrasso, Italy.
Jazyk: angličtina
Zdroj: Cell proliferation [Cell Prolif] 2024 Aug; Vol. 57 (8), pp. e13627. Date of Electronic Publication: 2024 Feb 29.
DOI: 10.1111/cpr.13627
Abstrakt: The central nervous system (CNS) is surrounded by three membranes called meninges. Specialised fibroblasts, originating from the mesoderm and neural crest, primarily populate the meninges and serve as a binding agent. Our goal was to compare fibroblasts from meninges and skin obtained from the same human-aged donors, exploring their molecular and cellular characteristics related to CNS functions. We isolated meningeal fibroblasts (MFs) from brain donors and skin fibroblasts (SFs) from the same subjects. A functional analysis was performed measuring cell appearance, metabolic activity, and cellular orientation. We examined fibronectin, serpin H1, β-III-tubulin, and nestin through qPCR and immunofluorescence. A whole transcriptome analysis was also performed to characterise the gene expression of MFs and SFs. MFs appeared more rapidly in the post-tissue processing, while SFs showed an elevated cellular metabolism and a well-defined cellular orientation. The four markers were mostly similar between the MFs and SFs, except for nestin, more expressed in MFs. Transcriptome analysis reveals significant differences, particularly in cyclic adenosine monophosphate (cAMP) metabolism and response to forskolin, both of which are upregulated in MFs. This study highlights MFs' unique characteristics, including the timing of appearance, metabolic activity, and gene expression patterns, particularly in cAMP metabolism and response to forskolin. These findings contribute to a deeper understanding of non-neuronal cells' involvement in CNS activities and potentially open avenues for therapeutic exploration.
(© 2024 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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