Stearoyl-CoA desaturase regulates organelle biogenesis and hepatic merozoite formation in Plasmodium berghei.

Autor: Narwal SK; Division of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, India., Mishra A; Division of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India., Devi R; Division of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India., Ghosh A; Division of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, India., Choudhary HH; Division of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, India., Mishra S; Division of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2024 May; Vol. 121 (5), pp. 940-953. Date of Electronic Publication: 2024 Feb 28.
DOI: 10.1111/mmi.15246
Abstrakt: Plasmodium is an obligate intracellular parasite that requires intense lipid synthesis for membrane biogenesis and survival. One of the principal membrane components is oleic acid, which is needed to maintain the membrane's biophysical properties and fluidity. The malaria parasite can modify fatty acids, and stearoyl-CoA Δ9-desaturase (Scd) is an enzyme that catalyzes the synthesis of oleic acid by desaturation of stearic acid. Scd is dispensable in P. falciparum blood stages; however, its role in mosquito and liver stages remains unknown. We show that P. berghei Scd localizes to the ER in the blood and liver stages. Disruption of Scd in the rodent malaria parasite P. berghei did not affect parasite blood stage propagation, mosquito stage development, or early liver-stage development. However, when Scd KO sporozoites were inoculated intravenously or by mosquito bite into mice, they failed to initiate blood-stage infection. Immunofluorescence analysis revealed that organelle biogenesis was impaired and merozoite formation was abolished, which initiates blood-stage infections. Genetic complementation of the KO parasites restored merozoite formation to a level similar to that of WT parasites. Mice immunized with Scd KO sporozoites confer long-lasting sterile protection against infectious sporozoite challenge. Thus, the Scd KO parasite is an appealing candidate for inducing protective pre-erythrocytic immunity and hence its utility as a GAP.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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