Concomitant [ 18 F]F-FAZA and [ 18 F]F-FDG Imaging of Gynecological Cancer Xenografts: Insight into Tumor Hypoxia.

Autor: Kepes Z; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; kepes.zita@med.unideb.hu., Hegedus E; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Sass T; Department of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Csikos C; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.; Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Szabo JP; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Szugyiczki V; Department of Nuclear Medicine, Békés County Pándy Kálmán Hospital, Semmelweis, Hungary., Hajdu I; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Kertesz I; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Opposits G; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Imrek J; Institute of Physics, University of Debrecen, Debrecen, Hungary., Balkay L; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary., Kalman FK; Department of Physical Chemistry, University of Debrecen, Debrecen, Hungary., Trencsenyi G; Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Jazyk: angličtina
Zdroj: In vivo (Athens, Greece) [In Vivo] 2024 Mar-Apr; Vol. 38 (2), pp. 574-586.
DOI: 10.21873/invivo.13476
Abstrakt: Background/aim: Herein we assessed the feasibility of imaging protocols using both hypoxia-specific [ 18 F]F-FAZA and [ 18 F]F-FDG in bypassing the limitations derived from the non-specific findings of [ 18 F]F-FDG PET imaging of tumor-related hypoxia.
Materials and Methods: CoCl 2 -generated hypoxia was induced in multidrug resistant (Pgp+) or sensitive (Pgp-) human ovarian (Pgp- A2780, Pgp+ A2780AD), and cervix carcinoma (Pgp- KB-3-1, Pgp+ KB-V-1) cell lines to establish corresponding tumor-bearing mouse models. Prior to [ 18 F]F-FDG/[ 18 F]F-FAZA-based MiniPET imaging, in vitro [ 18 F]F-FDG uptake measurements and western blotting were used to verify the presence of hypoxia.
Results: Elevated GLUT-1, and hexokinase enzyme-II expression driven by CoCl 2 -induced activation of hypoxia-inducible factor-1α explains enhanced cellular [ 18 F]F-FDG accumulation. No difference was observed in the [ 18 F]F-FAZA accretion of Pgp+ and Pgp- tumors. Tumor-to-muscle ratios for [ 18 F]F-FAZA measured at 110-120 min postinjection (6.2±0.1) provided the best contrasted images for the delineation of PET-oxic and PET-hypoxic intratumor regions. Although all tumors exhibited heterogenous uptake of both radiopharmaceuticals, greater differences for [ 18 F]F-FAZA between the tracer avid and non-accumulating regions indicate its superiority over [ 18 F]F-FDG. Spatial correlation between [ 18 F]F-FGD and [ 18 F]F-FAZA scans confirms that hypoxia mostly occurs in regions with highly active glucose metabolism.
Conclusion: The addition of [ 18 F]F-FAZA PET to [ 18 F]F-FGD imaging may add clinical value in determining hypoxic sub-regions.
(Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze: MEDLINE
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