Chemical components with antibacterial properties found in sanchen powder from traditional Tibetan medicine.

Autor: Zhao Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. Electronic address: ll2820064026@163.com., Xi C; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. Electronic address: xichenggjun@163.com., Liu D; Beijing Union Pharmaceutical Factory, Beijing, China. Electronic address: liudonghan80@163.com., Ren X; School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China; Institute of Minority Medicine, Beijing University of Chinese Medicine, Beijing, China. Electronic address: rxq23111111@126.com., Fan J; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. Electronic address: fanjiayi050800@163.com., Tangthianchaichana J; Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, Thailand. Electronic address: Jakkreetcm@hotmail.com., Lu Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. Electronic address: landocean28@163.com., Wu H; School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China; Institute of Minority Medicine, Beijing University of Chinese Medicine, Beijing, China. Electronic address: wuhuichao@bucm.edu.cn.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2024 May 23; Vol. 326, pp. 117981. Date of Electronic Publication: 2024 Feb 28.
DOI: 10.1016/j.jep.2024.117981
Abstrakt: Ethnopharmacological Relevance: Sanchen powder is a traditional Tibetan medicine comprising Bambusae Concretio Silicea, Carthami Flos, and Bovis Calculus Artifactus. Bambusae Concretio Silicea is the dried mass of secreted fluid in the stalks of Gramineae plants such as Bambusa textilis McClure or Schizostachyum chinense Rendle. Carthami Flos is the dried flower of Carthamus tinctorius L. in the Compositae plant. Bovis Calculus Artifactus is made from ox bile powder, cholic acid, hyodeoxycholic acid, taurine, bilirubin, cholesterol, and trace elements. Research has evidenced the antibacterial efficacy of Sanchen powder, albeit its active constituents for this effect are yet to be established.
Aim of the Study: To investigate effective compounds, potential targets, and molecular mechanism of Sanchen powder for its antibacterial properties by using network pharmacology combined with in vitro validation, with the aims of observing the action of effective compounds in Sanchen powder and exploring new therapeutic strategies for antibacterial.
Materials and Methods: In this study, UPLC-Q-TOF-MS was utilized to identify the chemical composition in Sanchen powder and its blood-borne chemical ingredients post-oral intake. A network pharmacology analysis was used to establish the chemical compound in the blood following oral administration-target-disease network. The study aimed to identify antibacterial active ingredients, which were then subjected to molecular docking and pharmacodynamic experiments to verify their efficacy.
Results: The findings demonstrate that following oral administration, the blood contains seven key components of Sanchen powder, including bilirubin, glycochenodeoxycholic acid, glycocholic acid, taurocholic acid, phenylalanine, safflomin A, and tryptophan. Additionally, the network pharmacology and molecular docking study results indicate the potential antibacterial effects of bilirubin, glycocholic acid, and glycochenodeoxycholic acid. In vitro antibacterial experiments revealed that bilirubin, glycocholic acid, and glycochenodeoxycholic acid could restrict the growth of the Staphylococcus aureus cell membrane at a certain concentration. Moreover, they exhibited antibacterial effects on Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Escherichia coli.
Conclusions: Bilirubin, glycocholic acid, and glycochenodeoxycholic acid could be effective therapeutic ingredients for the antibacterial effects of Sanchen powder. These results offer a foundation for further clinical application and research on the antibacterial effect of Sanchen powder, a Traditional Tibetan Medicine.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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