Novel Secreted Effectors Conserved Among Smut Fungi Contribute to the Virulence of Ustilago maydis .

Autor:	Schuster M; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany., Schweizer G; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.; Independent Data Lab UG, 80937 Munich, Germany., Reißmann S; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Happel P; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Aßmann D; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Rössel N; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Güldener U; Deutsches Herzzentrum München, Technische Universität München, 80636 München, Germany., Mannhaupt G; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Ludwig N; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.; Research & Development, Weed Control Bayer AG, Crop Science Division, 65926 Frankfurt am Main, Germany., Winterberg S; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Pellegrin C; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Tanaka S; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Vincon V; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Presti LL; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Wang L; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Bender L; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.; Department of Pharmaceutics and Biopharmaceutics, Phillips-University Marburg, 35037 Marburg, Germany., Gonzalez C; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany., Vranes M; Karlsruhe Institute of Technology, Institute for Applied Biosciences, Department of Genetics, 76131 Karlsruhe, Germany., Kämper J; Karlsruhe Institute of Technology, Institute for Applied Biosciences, Department of Genetics, 76131 Karlsruhe, Germany., Seong K; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, U.S.A., Krasileva K; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, U.S.A., Kahmann R; Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.
Jazyk:	angličtina
Zdroj:	Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2024 Mar; Vol. 37 (3), pp. 250-263. Date of Electronic Publication: 2024 Apr 03.
DOI:	10.1094/MPMI-09-23-0139-FI
Abstrakt:	Fungal pathogens deploy a set of molecules (proteins, specialized metabolites, and sRNAs), so-called effectors, to aid the infection process. In comparison to other plant pathogens, smut fungi have small genomes and secretomes of 20 Mb and around 500 proteins, respectively. Previous comparative genomic studies have shown that many secreted effector proteins without known domains, i.e., novel, are conserved only in the Ustilaginaceae family. By analyzing the secretomes of 11 species within Ustilaginaceae, we identified 53 core homologous groups commonly present in this lineage. By collecting existing mutants and generating additional ones, we gathered 44 Ustilago maydis strains lacking single core effectors as well as 9 strains containing multiple deletions of core effector gene families. Pathogenicity assays revealed that 20 of these 53 mutant strains were affected in virulence. Among the 33 mutants that had no obvious phenotypic changes, 13 carried additional, sequence-divergent, structurally similar paralogs. We report a virulence contribution of seven previously uncharacterized single core effectors and of one effector family. Our results help to prioritize effectors for understanding U. maydis virulence and provide genetic resources for further characterization. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu