The human PTGR1 gene expression is controlled by TE-derived Z-DNA forming sequence cooperating with miR-6867-5p.

Autor: Lee DH; Department of Integrated Biological Sciences, Pusan National University, Busan, 46241, Republic of Korea.; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea., Bae WH; Department of Integrated Biological Sciences, Pusan National University, Busan, 46241, Republic of Korea.; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea., Ha H; Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul, 03080, Republic of Korea., Kim WR; Department of Integrated Biological Sciences, Pusan National University, Busan, 46241, Republic of Korea.; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea., Park EG; Department of Integrated Biological Sciences, Pusan National University, Busan, 46241, Republic of Korea.; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea., Lee YJ; Department of Integrated Biological Sciences, Pusan National University, Busan, 46241, Republic of Korea.; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea., Kim JM; Department of Integrated Biological Sciences, Pusan National University, Busan, 46241, Republic of Korea.; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea., Shin HJ; Department of Integrated Biological Sciences, Pusan National University, Busan, 46241, Republic of Korea.; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea., Kim HS; Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea. khs307@pusan.ac.kr.; Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea. khs307@pusan.ac.kr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Feb 27; Vol. 14 (1), pp. 4723. Date of Electronic Publication: 2024 Feb 27.
DOI: 10.1038/s41598-024-55332-x
Abstrakt: Z-DNA, a well-known non-canonical form of DNA involved in gene regulation, is often found in gene promoters. Transposable elements (TEs), which make up 45% of the human genome, can move from one location to another within the genome. TEs play various biological roles in host organisms, and like Z-DNA, can influence transcriptional regulation near promoter regions. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that play a critical role in the regulation of gene expression. Although TEs can generate Z-DNA and miRNAs can bind to Z-DNA, how these factors affect gene transcription has yet to be elucidated. Here, we identified potential Z-DNA forming sequence (ZFS), including TE-derived ZFS, in the promoter of prostaglandin reductase 1 (PTGR1) by data analysis. The transcriptional activity of these ZFS in PTGR1 was confirmed using dual-luciferase reporter assays. In addition, we discovered a novel ZFS-binding miRNA (miR-6867-5p) that suppressed PTGR1 expression by targeting to ZFS. In conclusion, these findings suggest that ZFS, including TE-derived ZFS, can regulate PTGR1 gene expression and that miR-6867-5p can suppress PTGR1 by interacting with ZFS.
(© 2024. The Author(s).)
Databáze: MEDLINE
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