Generation of induced pluripotent stem cell line NIMHi010-A from dermal fibroblast cells of a healthy individual.

Autor:	Dash SS; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Arunachal G; Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India., Nimonkar MM; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Vengalil S; Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Nashi S; Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Chetan GK; Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India., Boddu VK; Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Nalini A; Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Markandeya YS; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India. Electronic address: yogananda@nimhans.ac.in.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2024 Apr; Vol. 76, pp. 103355. Date of Electronic Publication: 2024 Feb 23.
DOI:	10.1016/j.scr.2024.103355
Abstrakt:	In this study, we have established human induced pluripotent stem cell (hiPSC) line, NIMHi010-A of a 42-year-old healthy donor. The iPSC line was generated from human dermal fibroblasts using Sendai viruses carrying reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The generated hiPSC line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line will serve as a healthy control model for physiological processes and drug screening of Asian origin from Indian population. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu