Autophagy and oxidative stress modulation mediate Bortezomib resistance in prostate cancer.

Autor: Zafeiropoulou K; Division of Genetics, Cell Biology and Development, Department of Biology, University of Patras, Patras, Greece.; Hematology Division, Department of Internal Medicine, University of Patras Medical School-University Hospital, Patras, Greece., Kalampounias G; Division of Genetics, Cell Biology and Development, Department of Biology, University of Patras, Patras, Greece., Alexis S; Hematology Division, Department of Internal Medicine, University of Patras Medical School-University Hospital, Patras, Greece., Anastasopoulos D; Division of Genetics, Cell Biology and Development, Department of Biology, University of Patras, Patras, Greece., Symeonidis A; Hematology Division, Department of Internal Medicine, University of Patras Medical School-University Hospital, Patras, Greece., Katsoris P; Division of Genetics, Cell Biology and Development, Department of Biology, University of Patras, Patras, Greece.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Feb 27; Vol. 19 (2), pp. e0289904. Date of Electronic Publication: 2024 Feb 27 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0289904
Abstrakt: Proteasome inhibitors such as Bortezomib represent an established type of targeted treatment for several types of hematological malignancies, including multiple myeloma, Waldenstrom's macroglobulinemia, and mantle cell lymphoma, based on the cancer cell's susceptibility to impairment of the proteasome-ubiquitin system. However, a major problem limiting their efficacy is the emergence of resistance. Their application to solid tumors is currently being studied, while simultaneously, a wide spectrum of hematological cancers, such as Myelodysplastic Syndromes show minimal or no response to Bortezomib treatment. In this study, we utilize the prostate cancer cell line DU-145 to establish a model of Bortezomib resistance, studying the underlying mechanisms. Evaluating the resulting resistant cell line, we observed restoration of proteasome chymotrypsin-like activity, regardless of drug presence, an induction of pro-survival pathways, and the substitution of the Ubiquitin-Proteasome System role in proteostasis by induction of autophagy. Finally, an estimation of the oxidative condition of the cells indicated that the resistant clones reduce the generation of reactive oxygen species induced by Bortezomib to levels even lower than those induced in non-resistant cells. Our findings highlight the role of autophagy and oxidative stress regulation in Bortezomib resistance and elucidate key proteins of signaling pathways as potential pharmaceutical targets, which could increase the efficiency of proteasome-targeting therapies, thus expanding the group of molecular targets for neoplastic disorders.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Zafeiropoulou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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