Protamines and the sperm nuclear basic proteins Pandora's Box of insects.

Autor:	Leyden MR; Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA., Gowen B; Department of Biology, University of Victoria, Victoria, BC V8W 2Y2, Canada., Gonzalez-Romero R; Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada., Eirin-Lopez JM; Environmental Epigenetics Laboratory, Institute of Environment, Florida International University, Miami, FL, USA.; Florida International University, Miami, FL, USA., Kim BH; Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada., Hayashi F; Department of Biology, Tokyo Metropolitan University, Minamiosawa 1-1, Hachioji, Tokyo 192-0397, Japan., McCartney J; Institute of Natural Sciences, Massey University, Palmerston North, Manawatu, New Zealand., Zhang PC; W.M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges, Claremont, CA 91711, USA., Kubo-Irie M; Biological Laboratory, The Open University of Japan, Wakaba, Mihama-ku, Chiba, 261-8506, Japan., Shabanowitz J; Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA., Hunt DF; Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.; Department of Pathology, University of Virginia, Charlottesville, VA 22903, USA., Ferree P; W.M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges, Claremont, CA 91711, USA., Kasinsky H; Department of Zoology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada., Ausió J; Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada.
Jazyk:	angličtina
Zdroj:	Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2024 Jun 01; Vol. 102 (3), pp. 238-251. Date of Electronic Publication: 2024 Feb 26.
DOI:	10.1139/bcb-2023-0363
Abstrakt:	Insects are the largest group of animals when it comes to the number and diversity of species. Yet, with the exception of Drosophila , no information is currently available on the primary structure of their sperm nuclear basic proteins (SNBPs). This paper represents the first attempt in this regard and provides information about six species of Neoptera: Poecillimon thessalicus, Graptosaltria nigrofuscata, Apis mellifera, Nasonia vitripennis, Parachauliodes continentalis , and Tribolium castaneum . The SNBPs of these species were characterized by acetic acid urea gel electrophoresis (AU-PAGE) and high-performance liquid chromatography fractionated. Protein sequencing was obtained using a combination of mass spectrometry sequencing, Edman N-terminal degradation sequencing and genome mining. While the SNBPs of several of these species exhibit a canonical arginine-rich protamine nature, a few of them exhibit a protamine-like composition. They appear to be the products of extensive cleavage processing from a precursor protein which are sometimes further processed by other post-translational modifications that are likely involved in the chromatin transitions observed during spermiogenesis in these organisms. Competing Interests: All authors have read and agreed to the published version of the manuscript and there is no conflict of interest, including specific financial interest and relationships and affiliations relevant to the subject of the manuscript.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu