| Autor: |
Vera E; Centro de Estudios Científicos (CECs), Valdivia, Chile.; Universidad Austral de Chile, Valdivia, Chile., Cornejo I; Centro de Estudios Científicos (CECs), Valdivia, Chile.; Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia, Chile., Henao JC; Centro de Estudios Científicos (CECs), Valdivia, Chile.; Universidad Austral de Chile, Valdivia, Chile., Tribiños F; Centro de Estudios Científicos (CECs), Valdivia, Chile., Burgos J; Centro de Estudios Científicos (CECs), Valdivia, Chile., Sepúlveda FV; Centro de Estudios Científicos (CECs), Valdivia, Chile.; Facultad de Medicina y Ciencia, Universidad San Sebastián, Valdivia, Chile., Cid LP; Centro de Estudios Científicos (CECs), Valdivia, Chile.; Facultad de Medicina y Ciencia, Universidad San Sebastián, Valdivia, Chile. |
| Abstrakt: |
K + channel Kir7.1 expressed at the apical membrane of the retinal pigment epithelium (RPE) plays an essential role in retinal function. An isoleucine-to-threonine mutation at position 120 of the protein is responsible for blindness-causing vitreo-retinal dystrophy. We have studied the molecular mechanism of action of Kir7.1-I120T in vitro by heterologous expression and in vivo in CRISPR-generated knockin mice. Full-size Kir7.1-I120T reaches the plasma membrane but lacks any activity. Analysis of Kir7.1 and the I120T mutant in mixed transfection experiments, and that of tandem tetrameric constructs made by combining wild type (WT) and mutant protomers, leads us to conclude that they do not form heterotetramers in vitro. Homozygous I120T/I120T mice show cleft palate and tracheomalacia and do not survive beyond P0, whereas heterozygous WT/I120T develop normally. Membrane conductance of RPE cells isolated from WT/WT and heterozygous WT/I120T mice is dominated by Kir7.1 current. Using Rb + as a charge carrier, we demonstrate that the Kir7.1 current of WT/I120T RPE cells corresponds to approximately 50% of that in cells from WT/WT animals, in direct proportion to WT gene dosage. This suggests a lack of compensatory effects or interference from the mutated allele product, an interpretation consistent with results obtained using WT/- hemizygous mouse. Electroretinography and behavioral tests also show normal vision in WT/I120T animals. The hypomorphic ion channel phenotype of heterozygous Kir7.1-I120T mutants is therefore compatible with normal development and retinal function. The lack of detrimental effect of this degree of functional deficit might explain the recessive nature of Kir7.1 mutations causing human eye disease. NEW & NOTEWORTHY Human retinal pigment epithelium K + channel Kir7.1 is affected by generally recessive mutations leading to blindness. We investigate one such mutation, isoleucine-to-threonine at position 120, both in vitro and in vivo in knockin mice. The mutated channel is inactive and in heterozygosis gives a hypomorphic phenotype with normal retinal function. Mutant channels do not interfere with wild-type Kir7.1 channels which are expressed concomitantly without hindrance, providing an explanation for the recessive nature of the disease. |
