Evaluation of a microscale quantitative suspension test to determine the bactericidal and yeasticidal activity of glutaral - one step to improve sustainability in disinfectant testing.

Autor:	Gebel J; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany.; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany., Rausch M; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany.; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany., Bienentreu K; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany.; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany., Droop F; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany., Eggers M; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany.; Laboratory Prof. Gisela Enders MVZ GbR, Stuttgart, Germany., Gebel L; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany., Gemein S; Referenzinstitut für Bioanalytik, Bonn, Germany., Hornei B; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany.; Evangelisches Krankenhaus Oberhausen, Zentralbereich Krankenhaushygiene, Institut für Laboratoriumsmedizin und Klinische Mikrobiologie, Oberhausen., Ilschner C; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany.; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany., Jacobshagen A; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany.; Federal Institute of Drugs and Medical Devices (BfArM) - Medical Devices Division, Bonn, Germany., Kampf G; University Medicine Greifswald, Greifswald, Germany., Papan C; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany., Roesch K; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany.; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany., Schmitz L; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany., Suchomel M; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany.; Institute for Hygiene and Applied Immunology, Medical University Vienna, Medical-technical Hygiene, Vienna, Austria., Vossebein L; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany.; Hochschule Niederrhein - Fachbereich Textil- und Bekleidungstechnik, Mönchengladbach, Germany., Mutters NT; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany., Exner M; Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany.; VAH - Association for Applied Hygiene c/o Institute for Hygiene and Public Health, Bonn, Germany.
Jazyk:	angličtina
Zdroj:	GMS hygiene and infection control [GMS Hyg Infect Control] 2024 Jan 30; Vol. 19, pp. Doc03. Date of Electronic Publication: 2024 Jan 30 (Print Publication: 2024).
DOI:	10.3205/dgkh000458
Abstrakt:	Aims: To evaluate a newly developed microscale quantitative suspension test compared to the existing standard suspension test using determination of the bactericidal and yeasticidal activity of glutaral as one step to improve the sustainability of disinfectant testing. Methods: The testing principles of the quantitative suspension test according to VAH method 9 (comparable to EN 13727) was used as a standard suspension test using 8.0 mL product test solution, 1.0 mL organic load and 1.0 mL test suspension. In addition, a micro-scale suspension test was performed in 96-well plates with 160 µL product test solution, 20 µL organic load and 20 µL test suspension. S. aureus ATCC 6538, P. aeruginosa ATCC 15442 and C. albicans ATCC 10231 were test organisms. Glutaral was tested at concentrations of 0.05%, 0.1%, 0.2% and 0.3% with exposure times of 1, 5 and 15 min. Polysorbate 80 (30 g/L), lecithin (9 g/L), L-histidine (1 g/L) and glycine (10 g/L) were used as validated neutralizers. After serial dilution of the disinfectant-neutralizer-mixture, plates were incubated for 48 h at 36°C (bacteria) or 72 hours at 30°C ( C. albicans ) and colony forming units (cfu) counted. The lg reduction was calculated as the difference between the results of the water control and the disinfectant at the end of the exposure time. All experiments were done in triplicate under clean conditions. Means of lg reduction were compared with the unpaired t-test, p<0.05 was considered to be significant. Results: Sufficient bactericidal activity according the VAH test requirements of at least 5 lg was found with both methods in 16 data sets of 24 data sets in total, and insufficient bactericidal activity of less than 5 lg was found with both methods in 7 data sets. In one data set, the mean lg reduction was above 5 lg with the microscale method and <5 lg with the VAH method, with no significant difference between the data sets (p=0.3096; 0.2% glutaral, 1 min, P. aeruginosa ). A sufficient yeasticidal activity of at least 4 lg was found with both methods in one data set, an insufficient yeasticidal activity of less than 4 lg was found with both methods in 8 data sets. With one exception, no significant differences were detected between the two methods below the efficacy threshold. Conclusions: The microscale quantitative suspension test proved to provide results similar to those of VAH method 9 when the bactericidal and yeasticidal activity of glutaralwas evaluated, with 32 out of 33 evaluations yielding consistent results in terms of efficacy. Its suitability should be confirmed with additional bacterial species, additional biocidal active substances and in other laboratories. Competing Interests: Günter Kampf received honoraria from Schülke & Mayr, Germany, outside the submitted work. The other authors declare no conflict of interest. The views expressed here are those of the authors and do not necessarily reflect those of the universities or institutions they are affiliated with. (Copyright © 2024 Gebel et al.)
Databáze:	MEDLINE
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