Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities.

Autor: El-Naggar NE; Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El- Arab City, Alexandria, 21934, Egypt. nouraalahmady@yahoo.com., El-Sawah AA; Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt., Elmansy MF; Biotechnology and its Application Program, Department of Botany, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt., Elmessiry OT; Biotechnology and its Application Program, Department of Botany, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt., El-Saidy ME; Biotechnology and its Application Program, Department of Botany, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt., El-Sherbeny MK; Biotechnology and its Application Program, Department of Botany, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt., Sarhan MT; Biotechnology and its Application Program, Department of Botany, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt., Elhefnawy AA; Biotechnology and its Application Program, Department of Botany, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt., Dalal SR; Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Feb 25; Vol. 14 (1), pp. 4581. Date of Electronic Publication: 2024 Feb 25.
DOI: 10.1038/s41598-024-54698-2
Abstrakt: Gold nanoparticles (GNPs) are highly promising in cancer therapy, wound healing, drug delivery, biosensing, and biomedical imaging. Furthermore, GNPs have anti-inflammatory, anti-angiogenic, antioxidants, anti-proliferative and anti-diabetic effects. The present study presents an eco-friendly approach for GNPs biosynthesis using the cell-free supernatant of Streptomyces albogriseolus as a reducing and stabilizing agent. The biosynthesized GNPs have a maximum absorption peak at 540 nm. The TEM images showed that GNPs ranged in size from 5.42 to 13.34 nm and had a spherical shape. GNPs have a negatively charged surface with a Zeta potential of - 24.8 mV. FTIR analysis identified several functional groups including C-H, -OH, C-N, amines and amide groups. The crystalline structure of GNPs was verified by X-ray diffraction and the well-defined and distinct diffraction rings observed by the selected area electron diffraction analysis. To optimize the biosynthesis of GNPs using the cell-free supernatant of S. albogriseolus, 30 experimental runs were conducted using central composite design (CCD). The artificial neural network (ANN) was employed to analyze, validate, and predict GNPs biosynthesis compared to CCD. The maximum experimental yield of GNPs (778.74 μg/mL) was obtained with a cell-free supernatant concentration of 70%, a HAuCl 4 concentration of 800 μg/mL, an initial pH of 7, and a 96-h incubation time. The theoretically predicted yields of GNPs by CCD and ANN were 809.89 and 777.32 μg/mL, respectively, which indicates that ANN has stronger prediction potential compared to the CCD. The anticancer activity of GNPs was compared to that of doxorubicin (Dox) in vitro against the HeP-G2 human cancer cell line. The IC 50 values of Dox and GNPs-based treatments were 7.26 ± 0.4 and 22.13 ± 1.3 µg/mL, respectively. Interestingly, treatments combining Dox and GNPs together showed an IC 50 value of 3.52 ± 0.1 µg/mL, indicating that they targeted cancer cells more efficiently.
(© 2024. The Author(s).)
Databáze: MEDLINE
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