Genomic characterization of Moraxella bovis and Moraxella bovoculi Uruguayan strains isolated from calves with infectious bovine keratoconjunctivitis.

Autor:	Bilbao L; Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, PC 11600,, Montevideo, Uruguay., Acquistapace S; Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, PC 11600, Montevideo, Uruguay; Departamento de Investigación y Desarrollo, Virbac Uruguay SA, Millán 4175, PC 12900, Montevideo, Uruguay., Umpiérrez A; Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, PC 11600, Montevideo, Uruguay., Smircich P; Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, PC 11600,, Montevideo, Uruguay., Alonzo P; Departamento de Investigación y Desarrollo, Virbac Uruguay SA, Millán 4175, PC 12900, Montevideo, Uruguay., Sotelo-Silveira JR; Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, PC 11600,, Montevideo, Uruguay., Zunino P; Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, PC 11600, Montevideo, Uruguay. Electronic address: pzunino@iibce.edu.uy.
Jazyk:	angličtina
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2024 Apr-Jun; Vol. 56 (2), pp. 165-174. Date of Electronic Publication: 2024 Feb 24.
DOI:	10.1016/j.ram.2023.12.003
Abstrakt:	Infectious bovine keratoconjunctivitis (IBK) is an ocular disease that affects bovines and has significant economic and health effects worldwide. Gram negative bacteria Moraxella bovis and Moraxella bovoculi are its main etiological agents. Antimicrobial therapy against IBK is often difficult in beef and dairy herds and, although vaccines are commercially available, their efficacy is variable and dependent on local strains. The aim of this study was to analyze for the first time the genomes of Uruguayan clinical isolates of M. bovis and M. bovoculi. The genomes were de novo assembled and annotated; the genetic basis of fimbrial synthesis was analyzed and virulence factors were identified. A 94% coverage in the reference genomes of both species, and more than 80% similarity to the reference genomes were observed. The mechanism of fimbrial phase variation in M. bovis was detected, and the tfpQ orientation of these genes confirmed, in an inversion region of approximately 2.18kb. No phase variation was determined in the fimbrial gene of M. bovoculi. When virulence factors were compared between strains, it was observed that fimbrial genes have 36.2% sequence similarity. In contrast, the TonB-dependent lactoferrin/transferrin receptor exhibited the highest percentage of amino acid similarity (97.7%) between strains, followed by cytotoxins MbxA/MbvA and the ferric uptake regulator. The role of these virulence factors in the pathogenesis of IBK and their potential as vaccine components should be explored. (Copyright © 2024 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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