| Autor: |
Liao CP; Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China., Dong SQ; Institute of Radiation Medicine, Chinese Academy of Medical Sciences Tianjin 300192, China., Liu YL; Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China., Enirile; College of Mongolian Medicine and Pharmacy, Inner Mongolia Minzu University Tongliao 028000, China., Gao Y; Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China., Fan HR; Institute of Radiation Medicine, Chinese Academy of Medical Sciences Tianjin 300192, China., Cai QJ; Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences Beijing 100700, China., Dai JG; Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China., Tu Y; Chinese Medicine Research and Development Center, China Academy of Chinese Medical Sciences Beijing 100700, China. |
| Jazyk: |
čínština |
| Zdroj: |
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2024 Jan; Vol. 49 (2), pp. 509-517. |
| DOI: |
10.19540/j.cnki.cjcmm.20230728.201 |
| Abstrakt: |
This study investigated the absorption profile of Wuwei Qingzhuo San in different intestinal segments and the absorption characteristics of its alkaloids(piperine, piperanine, piperlonguminine, and dihydropiperlonguminine). The everted gut sac model was established, and the chemical components of Wuwei Qingzhuo San in different intestinal segments were detected by UPLC-Q-TOF-MS. The content of piperine, piperanine, piperlonguminine, and dihydropiperlonguminine in intestinal absorption fluid was determined by UPLC-Q-TRAP-MS and the absorption parameters were calculated. The absorption characteristics in different intestinal segments at different time were analyzed. As a result, 27, 27, 8, and 6 absorbent components from Wuwei Qingzhuo San were detected in the intestinal cyst fluid of jejunum, ileum, duodenum, and colon by UPLC-Q-TOF-MS technology, respectively. It was also found that piperine, piperanine, piperlonguminine, and dihydropiperlonguminine from Wuwei Qingzhuo San showed linear absorption in various intestinal segments, with r values exceeding 0.9. In terms of absorption content, the components were ranked as piperine>piperanine>dihydropiperlonguminine>piperlonguminine in various intestinal segments, but the absorption rate and mechanism of each component varied. The results demonstrate that the absorption of the components of Wuwei Qingzhuo San in different intestinal segments is selective and is not a simple semi-permeable membrane permeation process. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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